COLD-INDUCED AND POSTNATAL CHANGES IN BROWN ADIPOSE-TISSUE GANGLIOSIDE LEVELS
COLD-INDUCED AND POSTNATAL CHANGES IN BROWN ADIPOSE-TISSUE GANGLIOSIDE LEVELS
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DOI：
10.2170/jjphysiol.40.57
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发表时间：
1990-01-01
期刊：
影响因子：
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通讯作者：
OHNO, T
中科院分区：
文献类型：
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作者：
KUROSHIMA, A;OHNO, T
研究了低温和出生后发育对大鼠棕色脂肪组织（BAT）神经节苷脂水平的影响。肩胛间BAT先用冷丙酮脱脂，然后提取神经节苷脂。采用HPTLC法测定神经节苷类。发现BAT中主要的神经节苷脂为GM3。冷驯化大鼠（CA）和27代冷饲养大鼠每g新鲜组织重和无脂干物质中BAT GM3水平显著升高。4日龄和14日龄大鼠每克鲜重及无脂干物质中BAT GM3含量均显著高于12周龄成年动物。研究还表明，较高水平的BAT GM3与较高的BAT重量相对于体重有关。这些结果表明，BAT神经节苷脂，特别是GM3，与BAT的生长和/或功能有关，在冷诱导和出生后该组织的变化。
Effects of cold and postnatal development were studied on brown adipose tissue (BAT) ganglioside levels in rats. Interscapular BAT was defatted by cold acetone before ganglioside extraction. Gangliosides were determined using HPTLC. Major ganglioside in BAT was found to be GM3. BAT GM3 level per g of fresh tissue weight as well as fat-free dry matter significantly increased in cold-acclimated rats (CA) and in rats reared in cold for 27 generations. BAT GM3 level per g of fresh weight as well as fat-free dry matter was significantly higher in 4-day- and 14-day-old rats than that in adult animals (12 weeks old). It was also shown that the higher levels of BAT GM3 were associated with greater BAT weight relative to body weight. These results suggest that BAT ganglioside, especially GM3, is implicated in growth and/or function of BAT in terms of cold-induced and postnatal changes of this tissue.