ADHESION OF PARASITIZED RED-BLOOD-CELLS TO CULTURED ENDOTHELIAL-CELLS - A FLOW-BASED STUDY OF ISOLATES FROM GAMBIAN CHILDREN WITH FALCIPARUM-MALARIA
ADHESION OF PARASITIZED RED-BLOOD-CELLS TO CULTURED ENDOTHELIAL-CELLS - A FLOW-BASED STUDY OF ISOLATES FROM GAMBIAN CHILDREN WITH FALCIPARUM-MALARIA
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DOI：
10.1017/s0031182000067706
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发表时间：
1993-11-01
期刊：
影响因子：
2.4
通讯作者：
NASH, GB
中科院分区：
文献类型：
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作者：
COOKE, BM;MORRISJONES, S;NASH, GB
被寄生的红细胞粘附于血管内皮被认为在与严重恶性疟疾相关的缺血性并发症的发展中起重要作用。使用一种新的，基于流动的测定，我们已经调查了粘附的寄生红细胞福尔马林固定的人脐静脉内皮细胞（HUVEC），从32冈比亚受试者获得的分离株轻度或重度恶性疟疾。在生理相关的流动条件下，感染恶性疟原虫野生株的红细胞能够粘附于HUVEC，但粘附水平变化很大，范围为1至688个粘附细胞/mm（2）HUVEC。在分离株内，一些粘附的寄生细胞保持静止，而其他形成不太稳定的相互作用，并在细胞表面缓慢滚动。从轻度或重度疟疾病例中获得的分离株之间的寄生细胞粘附没有显着差异，尽管分离株的子集确实显示出非常高的粘附水平。结果表明，寄生细胞与培养的内皮细胞的粘附（可能是通过受体ICAM-1）和疾病的临床严重程度之间没有简单的关系，尽管体内微血管粘附的变化可能仍然是缺血性并发症的决定因素。
Adhesion of parasitized red blood cells to vascular endothelium is thought to play an important role in the development of the ischaemic complications associated with severe falciparum malaria. Using a novel, flow-based assay, we have investigated the adhesion of parasitized red blood cells to formalin-fixed human umbilical vein endothelial cells (HUVEC), for isolates obtained from 32 Gambian subjects with mild or severe falciparum malaria. Red cells infected with wild strains of Plasmodium falciparum were able to adhere to HUVEC under physiologically relevant flow conditions, but the level of adhesion was highly variable, ranging from 1 to 688 adherent cells per mm(2) of HUVEC. Within isolates, some adherent parasitized cells remained stationary, whilst other formed less stable interactions and rolled slowly over the cell surface. There was no significant difference in adhesion of parasitized cells between isolates obtained from mild or severe cases of malaria, although a subset of isolates did show very high levels of adhesion. The results suggest that there is not a simple relationship between the adhesion of parasitized cells to cultured endothelial cells (presumably via the receptor ICAM-1) and the clinical severity of the disease, although variation in microvascular adhesion in vivo may still be a determinant of ischaemic complications.