Immunoglobulin G response of periodontitis patients to Porphyromonas gingivalis capsular carbohydrate and lipopolysaccharide antigens.
Immunoglobulin G response of periodontitis patients to Porphyromonas gingivalis capsular carbohydrate and lipopolysaccharide antigens.
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牙周炎患者的免疫球蛋白 G 对牙龈卟啉单胞菌荚膜碳水化合物和脂多糖抗原的反应。
DOI：
10.1034/j.1399-302x.2001.160401.x
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发表时间：
2001
影响因子：
--
通讯作者：
Page,RC
中科院分区：
文献类型：
--
作者：
Sims,TJ;Schifferle,RE;Ali,RW;Skaug,N;Page,RC
参与牙周感染的牙龈卟啉单胞菌克隆型表达血清学上不同的表面抗原。本研究旨在确定血清抗体是否针对P的血清型特异性荚膜糖K抗原和脂多糖抗原滴度。牙龈炎可能揭示哪些血清型最有可能导致成人牙周炎患者的龈下感染。免疫球蛋白G（IgG）对纯化的K抗原和来自不同P.用ELISA法检测了28名健康对照者和51名牙周袋感染者的牙龈炎菌株。牙龈无性系型。测量所有受试者对来自分别代表血清型K1-K6的菌株W50、HG 184、A7A1 - 28、49417、HG 1690和HG 1691的纯化K抗原以及来自分别代表血清型O1-O3的菌株381、HG 1691和W50的脂多糖的滴度。使用卡方似然比、Mann-Whitney检验和受试者操作特征敏感性-特异性图比较不同靶抗原的滴度结果将受试者分为有或无疾病的准确性。靶向K2、K3、K4、K5、O1和O2的检测结果通常诊断准确性较差，无论是单独评价还是作为对应于靶抗原亲本菌株实际表达的K/O组合的总滴度对评价。O3（来自W50）和K5 + O2（均来自HG1690），在分类受试者时具有中等准确性。相比之下，使用单个K1（W50）和K6（HG1691）滴度数据以及K1 + O3（W50）和K6 + O2（HG1691）滴度总和值实现了高度显著的诊断准确性。这些结果表明P.表达与W50（K1/O3）和HG1691（K6/O2）血清型匹配的K/O血清型的牙龈单克隆型比其他类型更可能参与成年牙周炎患者的牙周感染，因此比其他类型更可能表达相关的毒力因子。
Porphyromonas gingivalisclonal types that participate in periodontal infections express serologically distinct surface antigens. This investigation sought to determine whether serum antibodies titers against the serotype‐specific capsular carbohydrate K antigen and lipopolysaccharide antigens ofP. gingivalismight reveal which serotypes are most likely to be responsible for subgingival infections in subjects with adult periodontitis. Immunoglobulin G (IgG) titers to purified K antigen and lipopolysaccharide from differentP. gingivalisstrains were measured by ELISA for 28 healthy controls and 51 patients with periodontal pockets known to be infected with genetically and serologically distinctP. gingivalisclonal types. Titers to purified K antigen from strains W50, HG184, A7A1–28, 49417, HG1690 and HG1691, representing serotypes K1‐K6, respectively, and lipopolysaccharide from strains 381, HG1691 and W50, representing serotypes O1–O3, respectively, were measured for all subjects. Chi‐square likelihood ratios, Mann‐Whitney tests and receiver‐operating characterstic sensitivity–specificity plots were used to compare the accuracy with which titer results for different target antigens classified subjects with or without disease. Results from assays targeting K2, K3, K4, K5, O1 and O2 generally gave poor diagnostic accuracy, whether evaluated separately or as summed titer pairs corresponding to the K/O combinations actually expressed by the target antigen parent strains. Exceptions were O3 (from W50) and K5+O2 (both from HG1690), which gave moderate accuracy in classifying subjects. In contrast, highly significant diagnostic accuracy was achieved using individual K1 (W50) and K6 (HG1691) titer data and K1+O3 (W50) and K6+O2 (HG1691) titer sum values. These observations suggest thatP. gingivalisclonal types expressing K/O serotypes matching those of W50 (K1/O3) and HG1691 (K6/O2) are more likely than others to participate in periodontal infections in adult periodontitis patients and thus are more likely than others to express relevant virulence factors.