Screening of KHP30-like prophages among Japanese Helicobacter pylori strains, and genetic analysis of a defective KHP30-like prophage sequence integrated in the genome of the H. pylori strain NY40.
Screening of KHP30-like prophages among Japanese Helicobacter pylori strains, and genetic analysis of a defective KHP30-like prophage sequence integrated in the genome of the H. pylori strain NY40.
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在日本幽门螺杆菌菌株中筛选 KHP30 样前噬菌体，并对整合到幽门螺杆菌 NY40 菌株基因组中的缺陷 KHP30 样前噬菌体序列进行遗传分析。
DOI：
10.1093/femsle/fnw157
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Matsuzaki S.
中科院分区：
文献类型：
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作者：
Uchiyama J;Takemura-Uchiyama I;Kato S;Takeuchi H;Sakaguchi Y;Ujihara T;Daibata M;Shimakura H;Okamoto N;Sakaguchi M;Matsuzaki S.
我们最近报道了活性幽门螺杆菌噬菌体（Hp），KHP 30和KHP 40，其基因组DNA在日本分离的宿主细菌菌株中以游离体形式存在（即假溶源性）。在这项研究中，我们研究了日本人中活性KHP 30样酶溶原性的可能性。pyloristries，因为它们的基因组含有一个假定的整合酶基因。在174株日本血吸虫中，只有NY40株在PCR中部分检测到KHP 30样原噬菌体序列。pylorisolates，除了产生上述活性菌株。接着，根据NY40菌株的基因组分析，发现KHP 30样原噬菌体序列位于ca.在宿主染色体中的524至549 kb。在NY40基因组中的附着位点attLandattR显示出与在法国分离株B38中检测到的几乎相同的基因组位置和序列，这表明活性亲本KHP 30样噬菌体以位点特异性方式整合到祖先NY40基因组中。在NY40基因组中发现的原噬菌体被认为是在位点特异性整合后被遗传修饰的。这些，连同其他的KHP 30样原噬菌体的数据。pylorigenomes，表明KHP 30样大肠杆菌的溶原状态通常是不稳定的。
We have recently reported the activeHelicobacter pyloribacteriophages (phages), KHP30 and KHP40, the genomic DNAs of which exist as episomes in host bacterial strains isolated in Japan (i.e. pseudolysogeny). In this study, we examined the possibility of the lysogeny of active KHP30-like phages in JapaneseH. pyloristrains, because their genomes contain a putative integrase gene. Only the NY40 strain yielded partial detection of a KHP30-like prophage sequence in PCR among 174 JapaneseH. pyloriisolates, except for strains producing the above active phages. Next, according to the genomic analysis of the NY40 strain, the KHP30-like prophage sequence was found to be located from ca. 524 to 549 kb in the host chromosome. The attachment sites,attLandattR, in the NY40 genome showed almost the same genomic location and sequence as those detected in a French isolate B38, suggesting that an active parental KHP30-like phage had integrated into the ancestral NY40 genome in a site-specific manner. The prophage found in the NY40 genome was assumed to have been genetically modified, after site-specific integration. These, together with the data in the KHP30-like prophages of otherH. pylorigenomes, suggest that the lysogenic state of the KHP30-like phages is generally unstable.