DNA binding provides a signal for phosphorylation of the RNA polymerase II heptapeptide repeats.
DNA binding provides a signal for phosphorylation of the RNA polymerase II heptapeptide repeats.
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DNA 结合为 RNA 聚合酶 II 七肽重复序列的磷酸化提供信号。
DOI：
10.1101/gad.6.3.426
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发表时间：
1992
影响因子：
10.5
通讯作者：
Dynan,WS
中科院分区：
文献类型：
--
作者：
Peterson,SR;Dvir,A;Anderson,CW;Dynan,WS
分离的转录复合物含有蛋白激酶，其以明显的启动子依赖性方式磷酸化RNA聚合酶II（RNAP II）大亚基的羧基末端结构域（CTD）的七肽重复。我们现在表明，该反应的基本特征可以在含有三个大分子组分的重构系统中再现：由融合到异源DNA结合结构域的RNAP II的CTD组成的融合蛋白，含有融合蛋白的识别序列的活化DNA片段，和非特异性结合DNA的蛋白激酶。这种激酶非常类似于以前已知的DNA依赖性蛋白激酶。显然，CTD与DNA的结合提供了磷酸化的关键信号。与其他DNA结合的转录因子的特异性接触似乎没有绝对的要求。
Isolated transcription complexes contain a protein kinase that phosphorylates the heptapeptide repeats of the carboxy-terminal domain (CTD) of the RNA polymerase II (RNAP II) large subunit in an apparently promoter-dependent manner. We now show that the essential features of this reaction can be reproduced in a reconstituted system containing three macromolecular components: a fusion protein consisting of the CTD of RNAP II fused to a heterologous DNA-binding domain, an activating DNA fragment containing the recognition sequence for the fusion protein, and a protein kinase that binds nonspecifically to DNA. This kinase closely resembles a previously known DNA-dependent protein kinase. Evidently, the association of the CTD with DNA provides a key signal for phosphorylation. There appears to be no absolute requirement for specific contacts with other DNA-bound transcription factors.