Elevated levels of adhesion molecules derived from leukocytes and endothelial cells in patients with pregnancy-induced hypertension
Elevated levels of adhesion molecules derived from leukocytes and endothelial cells in patients with pregnancy-induced hypertension
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DOI：
10.1081/prg-120016793
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发表时间：
2003-01-01
影响因子：
1.5
通讯作者：
Kameda, K
中科院分区：
文献类型：
--
作者：
Abe, E;Matsubara, K;Kameda, K
目标。我们的目的是阐明粘附分子在妊娠高血压（PIH）发病机制中的作用。方法。收集PIH患者、正常孕妇和非孕妇的血清、外周血淋巴细胞和多形核细胞（PMN）。ELISA法检测血清中可溶性细胞间粘附分子-1 （sICAM-1）的含量。内皮细胞（EC）上ICAM-1的表达。采用流式细胞术和RT-PCR对血清进行分析。流式细胞术检测淋巴细胞和PMN上CD11a、CD11b和CD18的表达。结果。PIH患者PMN和淋巴细胞CD11a、CD18表达水平显著高于正常孕妇（p < 0.05）。正常妊娠组CD11b表达明显高于非妊娠组（p < 0.05）。PIH患者血清sICAM-1水平高于正常孕妇（p < 0.05）。与PIH血清孵育24小时的EC中ICAM-1表达水平显著高于正常妊娠血清（p < 0.0005）。与正常孕妇血清相比，与PIH血清孵育12小时后，ICAM-1 mRNA表达也显著升高（p < 0.05）。结论。黏附分子可能在PIH的发病机制中起重要作用。
Objective. Our purpose was to elucidate the role of adhesion molecules in the pathogenesis of pregnancy-induced hypertension (PIH). Methods. Sera, peripheral lymphocytes, and polymorphonuclear cells (PMN) from PIH patients, normal pregnant women, and nonpregnant women were collected. Soluble intercellular adhesion molecule-1 (sICAM-1) in sera was measured by ELISA. ICAM-1 expression, on endothelial cells (EC) incubated with. sera was analyzed by flow cytometry and RT-PCR. CD11a, CD11b, and CD18 expression on lymphocytes and PMN were also measured by flow cytometory. Results. CD11a and CD18 expression levels on PMN and lymphocytes of PIH patients were significantly' higher than those of normal pregnant women (p < 0.05). The expression of CD11b was significantly increased in normal pregnancy compared with that in nonpregnant women (p < 0.05). Serum sICAM-1 in PIH patients was higher than that in normal pregnant women (p < 0.05). ICAM-1 expression level on EC incubated with PIH serum for 24 hr was significantly higher than that with normal pregnant serum (p < 0.0005). ICAM-1 mRNA expression after 12 hr incubation with PIH serum was also significantly increased compared with serum from normal pregnant women (p < 0.05). Conclusion. Adhesion molecules may play an important role in the pathogenesis of PIH.