Open tubular capillary electrochromatography using capillaries coated with films of alkanethiol-self-assembled gold nanoparticle layers
Open tubular capillary electrochromatography using capillaries coated with films of alkanethiol-self-assembled gold nanoparticle layers
复制标题
DOI：
10.1016/j.chroma.2005.06.035
复制
发表时间：
2005-08-12
影响因子：
4.1
通讯作者：
Wu, CH
中科院分区：
文献类型：
--
作者：
Liu, FK;Hsu, YT;Wu, CH
我们采用烷硫醇自组装和二硫醇层层自组装（LBL）工艺制备了一种纳米颗粒（NP）包被的开管毛细管电色谱（OTCEC）柱，用于分离三种中性类固醇药物（睾酮、孕酮和丙酸睾酮）。通过在3-氨基丙基三甲氧基硅烷（APTMS）修饰的熔融硅毛细管上LBL自组装An NPs，然后通过烷硫醇自组装An NPs的表面功能化制备CEC柱。我们使用三种类固醇药物的“反相”测试混合物研究了所得金np包被CEC柱的电色谱特性。我们发现影响分离性能的关键因素是An NP层的数量、在Au NP上自组装的烷硫醇碳原子链的长度、有机改性剂的百分比和运行电解质的pH。本研究表明，烷硫醇和二硫醇在An NPs上的自组装为CEC分离提供了易于制备的固定相，其保留行为具有高度可控性和可重复性。我们相信我们的发现将有助于进一步研究纳米技术在分离科学中的应用。(c) 2005 Elsevier B.V.版权所有
We have used alkanethiol self-assembly and dithiol layer-by-layer (LBL) self-assembly processes to prepare an An nanoparticle (NP)-coated open tubular capillary electrochromatography (OTCEC) column for the separation of three neutral steroid drugs (testosterone, progesterone, and testosterone propionate). The CEC column was fabricated through LBL self-assembly of An NPs on a 3-aminopropyltrimethoxysilane (APTMS)-modified fused-silica capillary and subsequent surface functionalization of the An NPs through self-assembly of alkanethiols. We investigated the electrochromatographic properties of the resulting Au NP-coated CEC column using a "reversed phase" test mixture of three steroid drugs. We found that the key factors affecting the separation performance were the number of An NP layers, the length of the carbon-atom chain of the alkanethiol self-assembled on the Au NPs, the percentage of organic modifier, and the pH of the running electrolyte. This study reveals that the self-assembly of alkanethiols and dithiols onto An NPs provides stationary phases for CEC separation that are easy to prepare and whose retention behavior is highly controllable and reproducible. We believe that our findings will contribute to further studies of the application of nanotechnology to separation science. (c) 2005 Elsevier B.V. All rights reserved.