T-cell immunoglobulin- and mucin-domain-containing molecule 3 gene polymorphisms and prognosis of non-small-cell lung cancer
T-cell immunoglobulin- and mucin-domain-containing molecule 3 gene polymorphisms and prognosis of non-small-cell lung cancer
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DOI：
10.1007/s13277-012-0610-1
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发表时间：
2013-04-01
期刊：
影响因子：
--
通讯作者：
Li, Qinchuan
中科院分区：
文献类型：
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作者：
Bai, Jianwen;Li, Xiaoyan;Li, Qinchuan
肺癌是世界范围内的主要死亡原因。非小细胞肺癌（NSCLC）占这些病例的大多数。含T细胞免疫球蛋白和粘蛋白结构域的分子3（TIM-3）已被确定为负调控分子，并在免疫耐受中起关键作用。研究表明，TIM-3基因多态性与多种疾病相关。本研究的目的是调查TIM-3基因多态性是否与非小细胞肺癌的易感性相关。应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术检测432例NSCLC患者和466例健康对照者的TIM-3基因-1516G/T、-574G/T和+4259T/G多态性。结果显示，TIM-3 + 4259 TG基因型在病例组和对照组中的频率分别为10.9%和4.1%，携带+4259TG基因型的受试者患NSCLC的风险是野生型的2.81倍（P < 0.0001）。TIM-3基因-1516G/T和-574G/T多态性与NSCLC无相关性。此外，在分析携带TIM-3 + 4259 T/G多态性的NSCLC患者的生存时间时，携带+4259TG基因型的患者的生存时间显著短于野生型患者（15.2个月vs. 26.7个月，P = 0.007）。提示TIM-3基因多态性与非小细胞肺癌的易感性有关，可作为非小细胞肺癌的预后指标。
Lung cancer is the leading cause of death worldwide. Non-small-cell lung cancer (NSCLC) accounts for most of these cases. T-cell immunoglobulin- and mucin-domain-containing molecule 3 (TIM-3) has been established as a negative regulatory molecule and plays a critical role in immune tolerance. Studies have shown that polymorphisms in TIM-3 gene can be associated with various diseases. The aim of this study was to investigate whether polymorphisms in the TIM-3 gene were associated with susceptibility to NSCLC. Three polymorphisms in TIM-3 gene (-1516G/T, -574G/T, and +4259T/G) were identified by polymerase chain reaction-restriction fragment length polymorphism in 432 NSCLC patients and 466 healthy controls. Results showed that frequencies of TIM-3 +4259TG genotype for cases and controls were 10.9 and 4.1 %, respectively; subjects carrying the +4259TG genotype had a 2.81-fold increased risk of NSCLC compared to the wild-type genotype (P < 0.0001). The TIM-3 -1516G/T and -574G/T polymorphisms did not show any correlation with NSCLC. In addition, when analyzing the survival time of NSCLC patients with TIM-3 +4259T/G polymorphism, cases with +4259TG genotype had significantly shorter survival time compared to the wild-type patients (15.2 months vs. 26.7 months, P = 0.007). These results suggested polymorphism in TIM-3 gene is associated with increased susceptibility to NSCLC and could be used as prognostic factor for this malignancy.