PROMOTION OF EMBRYONIC CHICK LIMB CARTILAGE DIFFERENTIATION BY TRANSFORMING GROWTH FACTOR-BETA
PROMOTION OF EMBRYONIC CHICK LIMB CARTILAGE DIFFERENTIATION BY TRANSFORMING GROWTH FACTOR-BETA
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DOI：
10.1016/0012-1606(89)90191-7
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发表时间：
1989-10-01
影响因子：
2.7
通讯作者：
KOSHER, RA
中科院分区：
文献类型：
--
作者：
KULYK, WM;RODGERS, BJ;KOSHER, RA
该研究代表了研究转化生长因子-β可能参与的第一步。（TGF-β）在鸡胚肢软骨分化的调控中的作用。TGF-β 1和2（1-10 ng/ml）引起阿辛蓝，pH 1阳性软骨基质，以及通过从整个第23/24期细胞制备的高密度微团培养物，35 S-硫酸盐-或3 H-葡糖胺-标记的硫酸化糖胺聚糖（GAG）的积累相应增加两倍至三倍。24肢芽或包括第25期翅芽的远侧亚脊间充质的成钟前体细胞的同质群体。此外，TGF-β.导致软骨特征性II型胶原蛋白和软骨特异性蛋白聚糖核心蛋白的mRNA的稳态细胞质水平显著增加（三倍至六倍）。仅短暂（2小时）暴露于TGF-β。在培养开始时，生长因子足以刺激软骨形成，表明生长因子在该过程的早期步骤起作用。此外，TGF-β.在肢体间充质细胞的低密度亚汇合点培养物中促进软骨基质的形成和软骨特异性基因表达，这是很少或没有软骨形成分化正常发生的情况。这些结果为考虑和进一步研究TGF-β的作用提供了强烈的动机。在控制肢体软骨分化方面的作用。
This study represents a first step in investigating the possible involvement of transforming growth factor-.beta. (TGF-.beta.) in the regulation of embryonic chick limb cartilage differentiation. TGF-.beta.1 amd 2 (1-10 ng/ml) elicit a striking increase in the accumulation of Alcian blue, pH 1-positive cartilage matrix, and a corresponding twofold to threefold increase in the accumulation of 35S-sulfate- or 3H-glucosamine-labelled sulfated glycosaminoglycans (GAG) by high density micromass cultures prepared from the cells of whole stage 23/24 limb buds or the homogeneous population of chrondrogenic precursor cells comprising the distal subridge mesenchyme of stage 25 wing buds. Moreover, TGF-.beta. causes a striking (threefold to sixfold) increase in the steady-state cytoplasmic levels of mRNAs for cartilage-characteristic type II collagen and the core protein of cartilage-specific proteoglycan. Only a brief (2 hr) exposure to TGF-.beta. at the initiation of culture is sufficient to stimulate chondrogenesis, indicating that the growth factor is acting at an early step in the process. Furthermore, TGF-.beta. promotes the formation of cartilage matrix and cartilage-specific gene expression in low density subconfluent spot cultures of limb mesenchymal cells, which are situations in which little, or no chondrogenic differentiation normally occurs. These results provide strong incentive for considering and further investigating the role of TGF-.beta. in the control of limb cartilage differentiation.