Association between p53 immunostaining and cigarette smoking in squamous cell carcinoma of the esophagus
Association between p53 immunostaining and cigarette smoking in squamous cell carcinoma of the esophagus
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DOI：
10.1093/jjco/hyd114
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发表时间：
2000-10-01
影响因子：
2.4
通讯作者：
Sasaguri, S
中科院分区：
文献类型：
--
作者：
Mizobuchi, S;Furihata, M;Sasaguri, S
背景：人们普遍认为吸烟与食管鳞癌密切相关。方法：74例食管鳞癌患者按每日吸烟量分为重度吸烟组(H组)26例、中度吸烟组(M组)39例和非吸烟组(N组)9例。用免疫组织化学方法比较三组间P53和视网膜母细胞瘤(Rb)的表达。结果：65.4%的H组、38.5%的M组和44.4%的N组P53基因表达阳性，H组阳性率显著高于M组(p=0.033)和M+N组(p=0.034)。RE过表达的频率差异无统计学意义。直接测序发现P53碱基突变有5种类型，最常见的是G：C到T：A颠换(35.3%)。结论：我们的研究提示吸烟的分子靶点之一是P53基因。P53点突变的模式涉及广泛的碱基对变化。
Background: it is generally accepted that cigarette smoking is closely associated with esophageal squamous cell carcinoma. This study investigated the molecular targets of cigarette smoke in carcinogenesis of the esophagus.Methods: Seventy-four patients with esophageal squamous cell carcinoma (SCC) were grouped according to daily cigarette consumption: heavy smoking group (group H) (n = 26), moderate smoking group (group M) (n = 39) and non-smoking group (group N) (n = 9). We compared p53 and retinoblastoma (RB) expression among the three groups by immunohistochemistry. In addition, fresh tumor tissues from 30 smokers with esophageal SCC were tested for p53 mutations in exons 5-8 by direct sequencing.Results: Staining for the p53 product was positive in 65.4% of group H, 38.5% of group M and 44.4% of group N. The frequency of positive staining in the group H was significantly higher than in group M (p = 0.033) and in group M + group N (p = 0.034). The difference with respect to the frequency of overexpression of RE was not significant. The patterns of p53 base-pair mutations in direct sequencing study were of five types, most commonly G:C to T:A transversion (35.3%).Conclusions: Our study suggests that one of the molecular targets of cigarette smoke is the p53 gene. The pattern of p53 point mutations involved a wide range of base-pair changes.