Reorientation of prostaglandin F secretion by calcium ionophore, estradiol, and prolactin in perifused porcine endometrium.
Reorientation of prostaglandin F secretion by calcium ionophore, estradiol, and prolactin in perifused porcine endometrium.
复制标题
灌注猪子宫内膜中钙离子载体、雌二醇和催乳素对前列腺素 F 分泌的重新定向。
作者：
T. Gross;M. Mirando;K. H. Young;S. Beers;F. Bazer;W. Thatcher
猪妊娠的建立需要子宫内膜前列腺素（PG）F分泌从内分泌（朝向子宫肌层和子宫血管系统）向外分泌（朝向子宫腔）方向转变。三个实验利用双侧子宫内膜灌注装置分别灌注子宫肌层和管腔表面，以确定是否促进钙循环跨管腔上皮表面，这可能是由雌二醇和PRL的相互作用引起的，是在建立妊娠猪的PGF分泌的重新定位。在实验1中，将发情后第14天来自周期性后备母猪（n = 7）的子宫内膜用盐水（对照）或添加到管腔表面灌注缓冲液中的钙离子载体A23187灌注。在实验2中，发情后第11天的周期母猪（n = 7）在单侧子宫切除术后接受戊酸雌二醇（EV）肌肉注射，并在EV后6 h（n = 3）或12 h（n = 4）切除剩余的EV刺激的子宫角。用含有250 ng/ml BSA或猪PRL的缓冲液灌注这些子宫内膜样品的两个表面。在实验3中，在发情后第14天采集周期性（n = 3）、妊娠（n = 4）和EV诱导假孕（n = 4）后备母猪的子宫内膜，并用BSA或PRL灌注至两侧表面。实验1和实验2中PGF的分泌方向最初为内分泌（P <0.01）。在实验1中，钙离子载体使PG分泌的方向由内分泌转向外分泌（P <0.01）。在实验2中，无论是EV在体内还是PRL在体外单独改变PGF分泌的方向。EV后6 h（P <0.01）和12 h（P <0.01），EV和PRL相互作用使PGF的分泌由内分泌转为外分泌。在实验3中，周期母猪的PGF分泌方向为内分泌，妊娠和假孕母猪的PGF分泌方向为外分泌（状态x侧; P <0.05）。PRL没有改变PGF分泌的方向，无论生殖状态。这些结果表明，在建立怀孕的猪子宫内膜PG分泌的重新定位涉及雌激素和PRL的相互作用，可能通过增加整个子宫上皮钙循环。
Establishment of pregnancy in pigs requires a shift in endometrial prostaglandin (PG) F secretion from an endocrine (toward the myometrium and uterine vasculature) to an exocrine (toward the uterine lumen) orientation. Three experiments utilized bilateral endometrial perfusion devices for separate perifusion of myometrial and luminal surfaces to determine whether promoting calcium cycling across the luminal epithelial surface, which may be induced by interactive effects of estradiol and PRL, is involved in the reorientation of PGF secretion during establishment of pregnancy in pigs. In Exp 1, endometrium from cyclic gilts (n = 7) on day 14 after estrus was perifused with either saline (control) or calcium ionophore A23187 added to luminal surface perifusion buffer. In Exp 2, cyclic gilts (n = 7) at day 11 after estrus received an im injection of estradiol valerate (EV) after unilateral hysterectomy and the remaining EV-stimulated uterine horn removed at 6 h (n = 3) or 12 h (n = 4) after EV. Both surfaces of these endometrial samples were perifused with buffer containing either 250 ng/ml BSA or porcine PRL. In Exp 3, endometrium from cyclic (n = 3), pregnant (n = 4), and EV-induced pseudopregnant (n = 4) gilts was collected on day 14 after estrus and perifused with BSA or PRL to both surfaces. Orientation of PGF secretion was initially endocrine in Exps 1 and 2 (P less than 0.01). In Exp 1, calcium ionophore shifted the orientation of PG secretion from endocrine to exocrine (P less than 0.01). In Exp 2, neither EV in vivo nor PRL in vitro alone altered the orientation of PGF secretion. However, EV and PRL interacted to reorient secretion of PGF from endocrine to exocrine at 6 h (P less than 0.01) and 12 h (P less than 0.01) after EV. In Exp 3, the orientation of PGF secretion was endocrine in cyclic gilts and exocrine in pregnant and pseudopregnant gilts (status x side; P less than 0.05). PRL did not alter the orientation of PGF secretion regardless of reproductive status. These results suggest that the reorientation of endometrial PG secretion in pigs during the establishment of pregnancy involves interactive effects of estrogens and PRL, possibly through increased calcium cycling across the uterine epithelium.