Optimizing a Noninvasive Oral Sampling Technique for Semicaptive Neotropical Primates in Peru
Optimizing a Noninvasive Oral Sampling Technique for Semicaptive Neotropical Primates in Peru
复制标题
优化秘鲁半圈养新热带灵长类动物的无创口腔采样技术
DOI：
10.7589/2018-10-248
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发表时间：
2020
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影响因子：
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通讯作者：
Marieke H Rosenbaum
中科院分区：
文献类型：
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作者：
Darby McDermott;A. P. Mendoza;Tierra Smiley;M. Zavaleta;A. Da’dara;J. Alarcón;Raul Bello;Paola Santa Vidal;Marieke H Rosenbaum
摘要：新热带灵长类动物（NP）的疾病监测受到在偏远地区麻醉NP采集样本的困难的限制。我们的目的是优化一种非侵入性的方法，从半自闭NP在秘鲁口腔采样。我们在秘鲁Madre de Dios的Taricaya救援中心提供了40 NP的绳索，这些绳索涂上了各种引诱剂，并测量了可能影响样品采集和质量的变量（绳索的接受度、咀嚼时间和从绳索中洗脱的液体体积）。我们采用液氮直接冷冻或室温RNA稳定试剂保存样品。通过使用常规PCR检测哺乳动物细胞色素b来测量样品的完整性。在82%（125/152）的试验中，NP成功地咀嚼了一根绳子。整体样品的完整性很高，96%（44/46）的样品（直接冷冻和储存在稳定试剂中）细胞色素b检测呈阳性。单个NP暴露于绳子程序的次数和NP年龄与较高的接受率和NP成功咀嚼绳子有关。我们的结论是，绳索是一种可行的非侵入性方法，可以在救援中心从NP中获取口腔样本，并可用于未来的研究，以评估群体遗传学和群体健康监测的病原体监测。
Abstract: Disease surveillance in Neotropical primates (NP) is limited by the difficulties associated with anesthetizing NP for sample collection in remote settings. Our objective was to optimize a noninvasive method of oral sampling from semicaptive NP in Peru. We offered 40 NP at Taricaya Rescue Centre in Madre de Dios, Peru ropes coated in various attractants and measured variables (acceptance of the rope, chewing time, and volume of fluid eluted from ropes) that may affect sample acquisition and quality. We preserved samples by direct freezing in liquid nitrogen or by storing samples in RNA stabilization reagent at room temperature. Sample integrity was measured by testing for mammalian cytochrome b with the use of conventional PCR. The NP successfully chewed on a rope in 82% (125/152) of trials. Overall sample integrity was high, with 96% (44/46) of samples (both directly frozen and stored in stabilization reagent) testing positive for cytochrome b. The number of times that an individual NP was exposed to the rope procedure and NP age were associated with higher acceptance rates and the NP successfully chewing on the rope. We conclude that ropes serve as a feasible noninvasive method of obtaining oral samples from NP at rescue centers and could be used in future studies to evaluate population genetics and for pathogen surveillance for population health monitoring.
生物物理学.J.45。
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