The Histone Deacetylases HosA and HdaA Affect the Phenotype and Transcriptomic and Metabolic Profiles of Aspergillus niger
The Histone Deacetylases HosA and HdaA Affect the Phenotype and Transcriptomic and Metabolic Profiles of Aspergillus niger
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组蛋白脱乙酰酶 HosA 和 HdaA 影响黑曲霉的表型、转录组和代谢谱
DOI：
10.3390/toxins11090520
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发表时间：
2019-09-01
期刊：
影响因子：
4.2
通讯作者：
Pan, Li
中科院分区：
文献类型：
--
作者：
Li, Xuejie;Pan, Lijie;Pan, Li
组蛋白乙酰化是调节染色质可及性的重要修饰，由两种组蛋白修饰酶控制：组蛋白乙酰化转移酶(HATS)和组蛋白去乙酰化酶(HDACs)。在丝状真菌中，越来越多的证据表明，HATS和HDAC是与菌丝生长、胁迫反应、致病性和次生代谢物(SM)产生相关的关键因素。本研究构建了7株组蛋白脱乙酰酶缺陷型黑曲霉菌株，研究了它们对菌株生长表型以及次生代谢途径转录和代谢的影响。表型分析表明，黑曲霉FGSC A1279中HOSA基因的缺失导致菌体生长、产色素、产孢量和抗逆性显著降低，hdaA基因缺失导致CD液体培养基色素产量增加。根据代谢组学分析，HOSA和hdaA突变体的次生代谢物伏马菌素的产量均减少，hdaA突变体的曲酸产量减少，HOSA突变体的曲酸产量略有增加。结果表明，组蛋白脱乙酰酶HOSA和HdaA在黑曲霉FGSC A1279的生长发育和SM生物合成中起重要作用。组蛋白脱乙酰酶为调节SM的合成提供了新的策略。
Histone acetylation is an important modification for the regulation of chromatin accessibility and is controlled by two kinds of histone-modifying enzymes: histone acetyltransferases (HATs) and histone deacetylases (HDACs). In filamentous fungi, there is increasing evidence that HATs and HDACs are critical factors related to mycelial growth, stress response, pathogenicity and production of secondary metabolites (SMs). In this study, seven A. niger histone deacetylase-deficient strains were constructed to investigate their effects on the strain growth phenotype as well as the transcriptomic and metabolic profiles of secondary metabolic pathways. Phenotypic analysis showed that deletion of hosA in A. niger FGSC A1279 leads to a significant reduction in growth, pigment production, sporulation and stress resistance, and deletion of hdaA leads to an increase in pigment production in liquid CD medium. According to the metabolomic analysis, the production of the well-known secondary metabolite fumonisin was reduced in both the hosA and hdaA mutants, and the production of kojic acid was reduced in the hdaA mutant and slightly increased in the hosA mutant. Results suggested that the histone deacetylases HosA and HdaA play a role in development and SM biosynthesis in A. niger FGSC A1279. Histone deacetylases offer new strategies for regulation of SM synthesis.