INDUCTION OF OBESITY IN RODENTS BY MEANS OF MONOSODIUM GLUTAMATE
INDUCTION OF OBESITY IN RODENTS BY MEANS OF MONOSODIUM GLUTAMATE
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DOI：
10.1079/bjn19760005
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发表时间：
1976-01-01
影响因子：
3.6
通讯作者：
SHAH, PP
中科院分区：
文献类型：
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作者：
BUNYAN, J;MURRELL, EA;SHAH, PP
1.以不同方法给不同年龄的小鼠和大鼠灌胃谷氨酸钠（MSG），观察其肥胖发生率.新生小鼠在1、2、3、6、7和8日龄皮下注射3 mg MSG/g体重，16%在断奶前死亡。在幸存者中，90%或更多的人变得明显肥胖。20-30周龄时，两种性别的平均屠体脂质含量均增加约120%。在雄性小鼠中，味精治疗增加体重和附睾脂肪垫重量，并大大减少肾上腺素刺激的脂肪分解分离的脂肪细胞。雌性动物的体重没有显著增加。从第13周至第15周，两种性别的摄食量均未增加。味精对小鼠血糖水平的影响不明显，但部分雄性小鼠血糖水平异常升高.从交配前3周到断奶，或从怀孕第14天到断奶，喂食100 g MSG/kg饲料的雌性小鼠的后代中未检测到肥胖.断奶时腹腔注射10毫克味精/克体重（分两次），到23周龄时，雌性小鼠的胴体脂肪含量增加了34%，但雌性大鼠没有受到影响。从断奶开始，在饮用水中添加20 g MSG/l不会增加雌性大鼠或小鼠的胴体脂质含量。从断奶开始，每公斤饲料添加20克味精，到14周龄时，雄性和雌性大鼠的体重或胴体脂质含量没有改变。与遗传性肥胖和金硫代葡萄糖（GTG）诱导的肥胖不同，MSG诱导的小鼠肥胖与暴食无关。研究的所有类型的小鼠，肥胖和瘦，具有基本相同的胴体水分含量和胴体脂肪含量之间的线性关系。虽然MSG肥胖小鼠不能通过体重增加而容易地与正常小鼠区分开，与遗传和GTG诱导的肥胖的50-120%相比，体重增加仅约10%，但是所提出的新生儿注射时间表在诱导高度肥胖方面几乎100%可靠。
1. Monosodium glutamate (MSG) was administered by various methods to mice and rats of various ages and the incidence of obesity was later measured.2. Newborn mice were injected subcutaneously with 3 mg MSG/g body-weight at 1, 2, 3, 6, 7 and 8 d of age; 16% died before weaning. Of the survivors, 90% or more became markedly obese. Mean carcass lipid content was increased by about 120% in both sexes at 20–30 weeks of age. In male mice, MSG treatment increased body-weight and epididymal fat pad weight, and greatly decreased adrenaline-stimulated lipolysis in isolated fat cells. Body-weight of females was not increased significantly. Food intake was not increased in either sex from weeks 13 to 15. Blood glucose level was not generally increased by MSG but some of the male mice had abnormally high values.3. Obesity was not detected in the offspring of female mice that had received 100 g MSG/kg diet, either from 3 weeks before mating until weaning, or from the 14th day of pregnancy until weaning.4. Intraperitoneal injection of 10 mg MSG/g body-weight (in two doses) at weaning increased carcass lipid content in female mice by 34% by 23 weeks of age, but female rats were not affected.5. The addition of 20 g MSG/l to the drinking-water from weaning onwards did not increase carcass lipid content in female rats or mice.6. The addition of 20 g MSG/kg diet from weaning onwards did not alter body-weight or carcass lipid content in male and female rats by 14 weeks of age.7. The obesity induced in mice by MSG was not associated with hyperphagia, unlike genetic obesity and obesity induced by gold thioglucose (GTG).8. All types of mouse studied, obese and lean, had essentially the same linear relationship between carcass water content and carcass lipid content.9. Although MSG-obese mice could not readily be differentiated from normal mice by the increase in body-weight, which was only about 10% compared to 50–120% for genetic and GTG-induced obesity, the proposed schedule of injections in the newborn was almost 100% reliable in inducing a high extent of adiposity.