Tunicamycin and Brefeldin A induce in plant cells a programmed cell death showing apoptotic features
Tunicamycin and Brefeldin A induce in plant cells a programmed cell death showing apoptotic features
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DOI：
10.1007/bf02680128
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发表时间：
2001-01-01
期刊：
影响因子：
2.9
通讯作者：
Bianchetti, R
中科院分区：
文献类型：
--
作者：
Crosti, P;Malerba, M;Bianchetti, R
最近在植物中发现的DAD基因表明，蛋白质的N-连锁糖基化可能是细胞程序性死亡计划的一个重要控制点。在本文中，我们描述衣霉素的作用。N连接蛋白糖基化抑制因子和高尔基体蛋白质转运抑制因子Brefeldin A对梧桐树(Acer Posoplatanus L.)细胞培养。事实证明，这两种化学物质能够强烈地加速细胞死亡：细胞和细胞核形态的变化；DNA片段化的增加，可通过特定的免疫反应检测到；以及DNA凝胶电泳中出现寡核小体大小的片段(梯状)。此外，Brefeldin A强烈刺激过氧化氢的产生，而不是衣霉素。这些结果也表明，在植物中，干扰内质网和高尔基体活动的化学物质强烈地诱导一种形式的程序性细胞死亡，表现出凋亡的特征。
The recent identification of DAD (defender against apoptotic death) gene in plants suggests that the N-linked glycosylation of proteins could be an important control point of plans programmed cell death. In this paper we describe the effects of Tunicamycin. an inhibitor of N-linked protein glycosylation, and Brefeldin A, an inhibitor of protein trafficking from the Golgi apparatus, on sycamore (Acer pseudoplatanus L.) cell cultures. These two chemicals proved able to induce a strong acceleration of the cell death: changes in cell and nucleus morphology; an increase in DNA fragmentation, detectable by a specific immunological reaction; and the presence of oligonucleosomal-size fragments (laddering) in DNA gel electrophoresis. Moreover, Brefeldin A, but not Tunicamycin, strongly stimulated the production of hydrogen peroxide. These results indicate that also in plants chemicals interfering with the activities of endoplasmic reticulum and of Golgi apparatus strongly induce a form of programmed cell death showing apoptotic features.