Maternal imprinting during mouse oocyte growth in vivo and in vitro.
Maternal imprinting during mouse oocyte growth in vivo and in vitro.
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DOI：
10.1016/j.bbrc.2009.07.131
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发表时间：
2009-10
影响因子：
3.1
通讯作者：
Zhenhua Song;Lingjiang Min;Qingjie Pan;Qinghua Shi;W. Shen
中科院分区：
文献类型：
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作者：
Zhenhua Song;Lingjiang Min;Qingjie Pan;Qinghua Shi;W. Shen
基因表达的表观遗传调控对哺乳动物的卵发生至关重要。本研究采用一种简单有效的方法从12.5dpc的培养胎鼠卵巢中获得卵母细胞。研究了这些卵母细胞的甲基化模式。结果表明，体外培养的胎鼠生殖细胞的卵母细胞中Igf2r和Peg3印迹的建立时间比体内卵母细胞慢。然而，卵母细胞在体外和体内具有相似的甲基化模式。Igf2r逐渐从头甲基化，在培养28天的卵母细胞中，甲基化覆盖80%的CpG位点。然而，在Peg3中只有45%的CpG位点在同一阶段被甲基化。此外，研究表明DNA甲基化程度与卵母细胞的大小在体内和体外均呈正相关，表明在卵母细胞生长过程中，DNA甲基化是一个渐进的过程。
Epigenetic regulation of gene expression is critical for oogenesis in mammals. In this study, a simple and efficient method was used to obtain the oocytes from cultured fetal mouse ovaries of 12.5dpc. The methylation pattern of these oocytes was examined. The results showed that the establishment of imprinting of Igf2r and Peg3 in oocytes derived from cultured fetal mouse germ cells in vitro follows a slower time course than that of oocytes in vivo. However, oocytes in vitro and in vivo share similar methylation patterns. Igf2r was gradually de novo methylated, and the methylation covers 80% CpG sites in oocytes cultured for 28days. However, only 45% of the CpG sites is methylated in Peg3 at the same stage. Furthermore, it demonstrated that the degree of DNA methylation is positively correlated with the size of oocytes in vitro and in vivo, indicating a progressive methylation process during oocyte growth.