Homeo domain of the yeast repressor alpha 2 is a sequence-specific DNA-binding domain but is not sufficient for repression.
Homeo domain of the yeast repressor alpha 2 is a sequence-specific DNA-binding domain but is not sufficient for repression.
复制标题
酵母抑制子 α2 的同源结构域是序列特异性 DNA 结合结构域，但不足以抑制。
DOI：
10.1126/science.2887035
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Johnson,AD
中科院分区：
文献类型：
--
作者：
Hall,MN;Johnson,AD
α-2蛋白是α-2基因的产物，是酿酒酵母细胞类型的调节因子。它通过与位于每个靶基因上游的操纵子结合来抑制一组细胞类型特异基因的转录。在α-2基因编码区构建了15个框内缺失。在体内检测缺失等位基因的表型，并在体外检测编码的突变蛋白与操作者的特异性结合能力。分析表明，α-2的序列特异性结合结构域位于68个氨基酸区域内。α2的这个区域与同源结构域有显著的同源性，同源结构域是在几个果蝇有丝分裂基因和片段基因的产物中发现的一个保守序列。此外，还有一类突变的α2蛋白在体外与操纵子紧密结合，但在体内无法抑制转录。
The α2 protein, the product of theMATα2 gene, is a regulator of cell type in the yeastSaccharomyces cerevisiae. It represses transcription of a group of cell type-specific genes by binding to an operator located upstream of each target gene. Fifteen in-frame deletions within the coding region of theMATα2 gene were constructed. The deletion alleles were examined for phenotypes conferred in vivo, and the encoded mutant proteins were assayed for ability to bind specifically to the operator in vitro. This analysis has revealed that the sequence-specific DNA-binding domain of α2 is located within a region of 68 amino acids. This region of α2 has significant homology with the homeo domain, a conserved sequence found in the products of severalDrosophilahomeotic and segmentation genes. In addition, there is a class of mutant α2 proteins that binds tightly and specifically to the operator in vitro, but fails to repress transcription in vivo.