Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88
Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88
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DOI：
10.1038/nbt1282
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发表时间：
2007-02-01
影响因子：
46.9
通讯作者：
Stam, Hein
中科院分区：
文献类型：
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作者：
Pel, Herman J.;de Winde, Johannes H.;Stam, Hein
丝状真菌尼日尔被发酵工业广泛用于生产酶和有机酸，特别是柠檬酸。我们对33.9兆碱基的A.尼日尔CBS 513.88，目前使用的酶生产菌株的祖先。高水平的同线性与其他曲霉测序观察。对识别的14，165个开放阅读框架中的6，506个进行了强功能预测。对蛋白质分泌途径的组成进行了详细的描述，并观察到曲霉的水解酶谱存在显著差异。一个由1,069个独特反应组成的重建代谢网络说明了A.尼日尔.值得注意的是大量的主要促进超家族转运蛋白和真菌锌双核簇转录因子，以及伏马菌素和赭曲霉毒素A合成的推定基因簇的存在。
The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid. We sequenced the 33.9-megabase genome of A. niger CBS 513.88, the ancestor of currently used enzyme production strains. A high level of synteny was observed with other aspergilli sequenced. Strong function predictions were made for 6,506 of the 14,165 open reading frames identified. A detailed description of the components of the protein secretion pathway was made and striking differences in the hydrolytic enzyme spectra of aspergilli were observed. A reconstructed metabolic network comprising 1,069 unique reactions illustrates the versatile metabolism of A. niger. Noteworthy is the large number of major facilitator superfamily transporters and fungal zinc binuclear cluster transcription factors, and the presence of putative gene clusters for fumonisin and ochratoxin A synthesis.