A highly sensitive protein-protein interaction assay based on Gaussia luciferase
A highly sensitive protein-protein interaction assay based on Gaussia luciferase
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DOI：
10.1038/nmeth979
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发表时间：
2006-12-01
期刊：
影响因子：
48
通讯作者：
Michnick, Stephen W.
中科院分区：
文献类型：
--
作者：
Remy, Ingrid;Michnick, Stephen W.
蛋白质片段互补测定 (PCA) 提供了研究体内和体外蛋白质-蛋白质相互作用动态的通用策略。 PCA 的全部潜力需要在亚内源蛋白表达水平上完全可逆且灵敏的检测。我们描述了一种符合这些标准的新测定法，该测定法基于高斯王子荧光素酶，证明了化学逆转以及连接胰岛素和 TGF beta 信号传导的关键相互作用的诱导和抑制。
Protein-fragment complementation assays (PCAs) provide a general strategy to study the dynamics of protein-protein interactions in vivo and in vitro. The full potential of PCA requires assays that are fully reversible and sensitive at subendogenous protein expression levels. We describe a new assay that meets these criteria, based on the Gaussia princeps luciferase enzyme, demonstrating chemical reversal, and induction and inhibition of a key interaction linking insulin and TGF beta signaling.