Detection of hepatitis C virus (HCV) in serum and peripheral-blood mononuclear cells from HCV-monoinfected and HIV/HCV-coinfected persons
Detection of hepatitis C virus (HCV) in serum and peripheral-blood mononuclear cells from HCV-monoinfected and HIV/HCV-coinfected persons
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DOI：
10.1086/430949
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发表时间：
2005-07-15
影响因子：
6.4
通讯作者：
Chung, RT
中科院分区：
文献类型：
--
作者：
Blackard, JT;Smeaton, L;Chung, RT
据推测，丙型肝炎病毒（HCV）在外周血单个核细胞（PBMCs）中复制，因此，这可能是与人类免疫缺陷病毒（HIV）相互作用的一个位点。我们采用链特异性实时聚合酶链反应检测了28例HCV单感染者和20例HIV/HCV合并感染者的HCV RNA。在第一次就诊时，89%的妇女血清样本中检测到HCV阳性链RNA，而分别在32%和55%的HCV单感染和HIV/HCV合并感染妇女的PBMC样本中检测到HCV阳性链RNA。在开始抗逆转录病毒治疗后，HIV/HCV合并感染的妇女在PBMC室中检测到阳性和阴性链HCV RNA的可能性明显高于HCV单一感染的妇女。HIV和HCV RNA水平不相关。血清HCV RNA水平随时间相关；血清和PBMC区室中的HCV RNA水平没有变化。这些数据表明HCV RNA在血清和PBMC区室中的调节存在差异，这可能部分解释了在合并感染者中观察到的有限的HCV抗病毒应答率。
It has been speculated that hepatitis C virus (HCV) replicates in peripheral-blood mononuclear cells (PBMCs), which, therefore, may be a site for interaction with human immunodeficiency virus (HIV). We used strand-specific real-time polymerase chain reaction to detect HCV RNA in 28 HCV-monoinfected and 20 HIV/HCV coinfected women. At the first visit, positive-strand HCV RNA was detected in serum samples from 89% of the women, whereas positive-strand HCV RNA was detected in PBMC samples from 32% and 55% of the HCV-monoinfected and HIV/HCV-coinfected women, respectively. After initiation of antiretroviral therapy, the HIV/HCV-coinfected women were significantly more likely to have detectable positive-and negative-strand HCV RNA in the PBMC compartment than were the HCV-monoinfected women. HIV and HCV RNA levels were not correlated. Serum HCV RNA levels were correlated over time; HCV RNA levels in the serum and PBMC compartments were not. These data suggest differential regulation of HCV RNA in the serum and PBMC compartments and may partially explain the limited HCV antiviral response rates observed in coinfected persons.