Fine mapping of the temperature-sensitive semi-dwarf (Tssd) locus regulating the internode length in peach (Prunus persica)
Fine mapping of the temperature-sensitive semi-dwarf (Tssd) locus regulating the internode length in peach (Prunus persica)
复制标题
调节桃（Prunus persica）节间长度的温度敏感半矮化（Tssd）基因座的精细定位
DOI：
10.1007/s11032-016-0442-6
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发表时间：
2016-02-01
影响因子：
3.1
通讯作者：
Wang, Zhiqiang
中科院分区：
文献类型：
--
作者：
Lu, Zhenhua;Niu, Liang;Wang, Zhiqiang
株高是果树树形结构的重要组成部分，是果树的重要农艺性状。降低树高有利于增加种植密度、提高产量、降低成本和延长果园寿命。然而，调节桃高度的遗传和分子因素尚不清楚。在这里，我们报告了一个半矮化桃突变体，它在低于30摄氏度的温度下表现出极度缩短的节间。遗传分析表明，该性状受一对显性基因控制，命名为温敏半矮秆（Tssd）。为了绘制Tssd基因座，两个DNA池，每个池由50个突变体或野生型同胞组成，进行特定长度的扩增片段测序。SLAF分析，随后使用桑格测序和高分辨率解链基因分型进行标记验证，基于Lovell的参考桃基因组序列，将Tssd基因定位在支架3中2.35和3.10 Mb之间跨越约750 kb的区域中。进一步的SNP被开发用于精细定位该基因座，该基因座跨越500 Kb的区间，包含69个预测的蛋白质编码基因模型。该区间两侧的SNP标记可用于Tssd个体的分子鉴定。这一结果将为温度如何调节温带果树节间长度提供信息。
As a key component of tree architecture, plant height is an important agronomic trait in fruit trees. Reducing tree height is beneficial for increasing planting density, allowing higher yields, lowering costs and increasing the orchard's lifespan. However, the genetic and molecular factors that regulate peach height are unknown. Here, we report a semi-dwarf peach mutant, which exhibits extremely shortened internodes at temperature below 30 degrees C. Genetic analysis indicated that this trait was controlled by a single dominant gene that we have named as temperature-sensitive semi-dwarf (Tssd). To map the Tssd locus, two DNA pools, each consisting of 50 mutant or wild-type siblings, were subjected to specific length amplified fragment sequencing. SLAF analysis followed by marker validation using Sanger sequencing and high-resolution melting genotyping located the Tssd gene in a region spanning approximately 750 kb between 2.35 and 3.10 Mb in scaffold 3 based on the reference peach genome sequence of 'Lovell.' Further SNPs were developed for fine mapping the locus, which spans an interval of 500 Kb and contains 69 predicted protein-coding gene models. The SNP markers flanking this interval can be applied in molecular identification of Tssd individuals. The result will provide information on how temperature regulates internode length in temperate fruit trees.