The chromogranin A-derived antifungal peptide CGA-N9 induces apoptosis in Candida tropicalis
The chromogranin A-derived antifungal peptide CGA-N9 induces apoptosis in Candida tropicalis
复制标题
嗜铬粒蛋白 A 衍生的抗真菌肽 CGA-N9 诱导热带假丝酵母细胞凋亡
DOI：
10.1042/bcj20190483
复制
发表时间：
2019-10-01
影响因子：
4.1
通讯作者：
Yang, Minghang
中科院分区：
文献类型：
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作者：
Li, Ruifang;Chen, Chen;Yang, Minghang
CGA-N9是一种来源于人嗜铬粒蛋白A（CGA）的肽，在我们以前的研究中发现具有抗菌活性，但其作用机制尚不清楚。在此，研究了CGA-N9的作用机制。我们发现，CGA-N9诱导细胞膜的去极化和钙离子的摄取进入细胞质和线粒体。随着线粒体膜电位的破坏，细胞内活性氧（ROS）的产生增加。因此，我们评估了CGA-N9处理后热带念珠菌细胞的凋亡过程，发现细胞色素c泄漏，染色质凝聚和DNA降解。CGA-N9与DNA的体外相互作用表明，CGA-N9不降解DNA，而是通过静电作用与DNA结合。综上所述，CGA-N9通过诱导C.热带植物
CGA-N9, a peptide derived from human chromogranin A (CGA), was found to have antimicrobial activity in our previous investigation, but its mechanism of action remains unclear. Herein, the mechanism of action of CGA-N9 was investigated. We found that CGA-N9 induced the depolarization of the cell membrane and uptake of calcium ions into the cytosol and mitochondria. With the disruption of the mitochondrial membrane potential, the generation of intracellular reactive oxygen species (ROS) increased. Accordingly, we assessed apoptotic processes in Candida tropicalis cells post-treatment with CGA-N9 and found cytochrome c leakage, chromatin condensation and DNA degradation. The interaction of CGA-N9 with DNA in vitro showed that CGA-N9 did not degrade DNA but bound to DNA via an electrostatic interaction. In conclusion, CGA-N9 exhibits antifungal activity by inducing apoptosis in C. tropicalis.