MOLECULAR-CLONING AND CHARACTERIZATION OF THE HTLV-III VIRUS ASSOCIATED WITH AIDS
MOLECULAR-CLONING AND CHARACTERIZATION OF THE HTLV-III VIRUS ASSOCIATED WITH AIDS
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DOI：
10.1038/312166a0
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发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
WONGSTAAL, F
中科院分区：
文献类型：
--
作者：
HAHN, BH;SHAW, GM;WONGSTAAL, F
我们最近报道了一种新的人类嗜T淋巴细胞逆转录病毒，HTLV-III，在获得性免疫缺陷综合征（AIDS）患者和疾病的风险1 -4的分离和表征。经过广泛的血清流行病学研究3，5，6，以及从这些患者中分离出的大量病毒1，7，我们得出结论，HTLV-III是艾滋病的病原体。在这里，我们报告的分子克隆和表征的两个高度相关，但不同形式的HTLV-III基因组。该病毒基因组是1000个酶长，并检测到在HTLV-III感染的细胞，但不是在未感染的细胞，包括正常的人体组织，表明这种病毒是外源性的man. We还证明了遥远的核酸序列之间的同源性克隆基因组的HTLV-III和那些HTLV-I和HTLV-II。克隆的HTLV-III基因组的可用性现在将允许将该病毒与也与AIDS的发病机制相关的其他逆转录病毒进行明确的比较8 -11，并且还将促进AIDS治疗中的诊断和治疗措施的发展。
We recently reported the isolation and characterization of a novel human T-lymphotropic retrovirus, HTLV-III, in patients with acquired immune deficiency syndrome (AIDS) and in those at risk for the disease1–4. After extensive sero-epidemiological studies3,5,6, together with numerous virus isolations from these patients1,7, we concluded that HTLV-III is the causative agent of AIDS. Here we report the molecular cloning and characterization of two highly related but distinct forms of the HTLV-III genome. The viral genome is ∼10 kilobases long and is detected in HTLV-III-infected cells but not in uninfected cells, including normal human tissue, indicating that this virus is exogenous to man. We also demonstrate distant nucleic acid sequence homology between the cloned genome of HTLV-III and those of HTLV-I and HTLV-II. The availability of the cloned HTLV-III genome will now allow an unambiguous comparison of this virus with other retroviruses that also have been associated with the pathogenesis of AIDS8–11, and moreover, will facilitate the development of diagnostic and therapeutic measures in the treatment of AIDS.