Growth factor messenger ribonucleic acid expression during differentiation of porcine embryonic myogenic cells.
Growth factor messenger ribonucleic acid expression during differentiation of porcine embryonic myogenic cells.
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猪胚胎肌原细胞分化过程中生长因子信使核糖核酸的表达。
DOI：
10.2527/jas.2006-351
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发表时间：
2007
影响因子：
3.3
通讯作者：
M. White
中科院分区：
文献类型：
--
作者：
G. Xi;M. Hathaway;W. Dayton;M. White
已知生长因子IGF-I和II及其结合蛋白IGFBP和转化生长因子（TGF）超家族成员（肌生长抑制素和TGFbeta1）调节肌源性细胞的增殖和分化。我们假设IGF和tgf β系统成员相对表达的变化在调节猪胚胎肌生成细胞（PEMC）培养的肌肉发生中起重要作用。因此，确定这些因子在PEMC肌生成过程中的表达模式是重要的。因此，我们使用real-time PCR来探索IGF-I的模式；IGF-II;IGFBP-2、-3和-5；IGF-type-I受体;myogenin;肌肉生长抑制素;和TGFbeta1 mRNA在PEMC肌肉发生过程中的表达。通过肌酸激酶活性和肌原素mRNA表达来评估分化的进展。正如预期的那样，肌酸激酶活性在48小时的PEMC培养中很低，在48小时和144小时达到峰值之间增加了20倍（P < 0.0001）。同样，肌生成素mRNA在48小时很低，随着分化的进展增加了大约5倍（P < 0.0001），在120小时达到峰值，在144小时下降。IGF-I和IGFBP-2 mRNA的表达模式相似，在48小时的PEMC培养中相对较低。增加大约5倍(P < 0.0001),在120 h的最大水平。相比之下,IGF-II和IGFBP-5 mRNA水平相对较高的48 h,达到72 h,并稳步减少60 - 80%,分别为(P < 0.001),在144 h。IGF-type-I受体信使rna水平是相对较高的h(直到96年文化,后下降了40% (P < 0.01),达到低水平在144 h。IGFBP-3 mRNA在48 h,相对较高的大约40%下降到最低水平在72 h (P < 0.001),然后增加大约60% (P < 0.001),他们最大的水平在144 h。TGFbeta1 mRNA水平下降约30% (P < 0.0001) 48至96 h,然后迅速反弹到一个高峰在120 h, h,到144年降至72年水平h。肌肉生长抑制素mRNA在48小时的最大水平,72年和96年之间下降迅速,最终减少约80%在144 h (P < 0.0001)。我们的数据表明，这些因子在PEMC肌肉发生过程中受到差异调节，并为培养的猪肌肉细胞中它们的mRNA表达模式提供了新的信息。
The growth factors, IGF-I and II, their binding proteins, IGFBP, and members of the transforming growth factor (TGF) superfamily (myostatin and TGFbeta1) are known to regulate proliferation and differentiation of myogenic cells. We hypothesized that changes in the relative expression of members of the IGF and TGFbeta systems play a significant role in regulating myogenesis in porcine embryonic myogenic cell (PEMC) cultures. Therefore, determining the expression patterns of these factors during PEMC myogenesis is important. Consequently, we used real-time PCR to explore the pattern of IGF-I; IGF-II; IGFBP-2, -3, and -5; IGF-type-I receptor; myogenin; myostatin; and TGFbeta1 mRNA expression during PEMC myogenesis. The progression of differentiation was assessed using creatine kinase activity and myogenin mRNA expression. As anticipated, creatine kinase activity was low in PEMC cultures at 48 h and increased 20-fold (P < 0.0001) between 48 h and its peak at 144 h. Similarly, myogenin mRNA was low at 48 h and increased approximately 5-fold (P < 0.0001) as differentiation progressed, peaking at 120 h and decreasing at 144 h. The patterns of IGF-I and IGFBP-2 mRNA expression were similar and were relatively lower in 48-h PEMC cultures, increasing approximately 5-fold (P < 0.0001) to their greatest levels at 120 h. In contrast, IGF-II and IGFBP-5 mRNA levels were relatively high at 48 h, peaking at 72 h, and steadily decreasing by 60 and 80%, respectively (P < 0.001), at 144 h. The level of IGF-type-I receptor mRNA was relatively high until 96 h of culture, after which it decreased 40% (P < 0.01), reaching a low at 144 h. Levels of IGFBP-3 mRNA were relatively high at 48 h, dropped approximately 40% to their lowest level at 72 h (P < 0.001), and then increased approximately 60% (P < 0.001) to their greatest levels at 144 h. Levels of TGFbeta1 mRNA decreased approximately 30% (P < 0.0001) between 48 and 96 h, then quickly rebounded to a peak at 120 h, and by 144 h had dropped to the levels seen at 72 h. Myostatin mRNA was at its greatest level at 48 h and declined rapidly between 72 and 96 h, finally decreasing by approximately 80% at 144 h (P < 0.0001). Our data demonstrate that these factors are differentially regulated during PEMC myogenesis and provide new information about their pattern of mRNA expression in cultured porcine muscle cells.
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DOI：
10.1016/s0021-9258(18)98496-6
发表时间：
1991-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Florini;K. Magri;D. Ewton;P. James;K. Grindstaff;P. Rotwein
通讯作者：
J. Florini;K. Magri;D. Ewton;P. James;K. Grindstaff;P. Rotwein
肌肉发育中的胰岛素样生长因子（IGF）。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Tollefsen,SE;Lajara,R;McCusker,RH;Clemmons,DR;Rotwein,P
通讯作者：
Rotwein,P
BC3H-1 肌细胞中胰岛素样生长因子 (IGF)-II 诱导 IGF-I 受体下调的机制。
DOI：
10.1677/joe.0.1410069
发表时间：
1994
期刊：
The Journal of endocrinology
影响因子：
--
作者：
Rosenthal,SM;Brown,EJ
通讯作者：
Brown,EJ
成肌细胞分化过程中胰岛素样生长因子结合蛋白5基因转录的快速激活。
DOI：
10.1210/mend.9.7.7476973
发表时间：
1995
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
--
作者：
Rotwein,P;James,PL;Kou,K
通讯作者：
Kou,K
体外肌肉细胞分泌的胰岛素样生长因子结合蛋白类型的鉴定。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
McCusker,RH;Camacho-Hübner,C;Clemmons,DR
通讯作者：
Clemmons,DR