Mechanism of Plant-Cell Damage in an Electrostatic Field
Mechanism of Plant-Cell Damage in an Electrostatic Field
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作者：
L. Murr
Irecentl Y1提出了几个关于模拟电场环境对植物生长的生理效应的发现。在这篇早期文章中得出的结论是，一种可能的机制可以解释观察到的效果，例如，广泛的细胞和组织损伤，以及活化植物的绿色着色，是电离。一种涉及极化盐迁移的渗透现象，通过灾难性渗透作用引发细胞破裂。然而，本文的文本将表明，这一原始假设的主要部分是不正确的，并且A的真正基础。损伤机制植根于涉及细胞代谢的酶成分的生化转化。如me 1· 2先前所述，用幼苗果园草进行实验。在该实验中，将相对电位梯度建立在30、50和75 kVjm。在每个参考电位梯度下进行两个生长重复;每个实验的生长期与先前工作中通过rne 1· 2建立的相同，即在15-h日长下为4周。这些剪报然后被归类为。每个电位梯度参考水平的单个样品，并干燥。然后将每个样品的75%灰化用于mll。ss光谱分析，而
Irecentl Y 1 presented several findings conceming the physiological effects on plant growth of a simulated electric-field environment. It was concluded in this earlier article that a possible mechanism which would explain the effects observed, for example, extensive cell and tissue damage, and a green coloration of the activated plants, was an ioniza. tion phenomenon involving the migration of polarized salts which initiated cell bursting by catastrophic osmosis. The text of the present article will show, however, that the major portion of this original hypothesis was incorrect, and that the true basis for a. damage mechanism is rooted in a biochemical transformation involving enzyme constituents of cell metabolism. Experiments with seedling orchard grass were conducted as described previously by me1• 2• Relative potential gradients in this experiment were established at 30, 50, and 75 kVjm. Two growth replications were conducted at each reference potential gradient; and the growing period of each experiment was identical to that established in previous work by rne1• 2, that is, 4 weeks at 15-h day-length.Leaf samples were then clipped from both the active and control environments of each experiment after the first two weeks of growth, and after each week following the initial clippings. These clippings were then grouped into a. single sample for each potential gradient referencelevel, and dried. Seventy-five per cent of each sample was then ashed for mll. ss spectroscopic analysis, while the