Localization of RAP1 and topoisomerase II in nuclei and meiotic chromosomes of yeast.
Localization of RAP1 and topoisomerase II in nuclei and meiotic chromosomes of yeast.
复制标题
Rap1和拓扑异构酶II在酵母的核和减数分裂染色体中的定位。
DOI：
10.1083/jcb.117.5.935
复制
发表时间：
1992-06
影响因子：
7.8
通讯作者：
Gasser, S M
中科院分区：
文献类型：
--
作者：
Klein, F;Laroche, T;Cardenas, M E;Hofmann, J F;Schweizer, D;Gasser, S M
拓扑异构酶II（topoII）和RAP 1（阻遏激活蛋白1）是两种丰富的核蛋白，在染色体的高级组织中具有拟议的结构作用。这两种蛋白共同fractionate作为从植物生长的酵母细胞的核支架的组件，并且这两种蛋白作为粗线期染色体的组件存在，与减数分裂核的不溶性亚组分共分馏。免疫定位使用特异性topoII的抗体显示染色的酵母减数分裂染色体的轴向核心，延长联会复合体的长度。另一方面，RAP 1位于配对的二价染色体的末端，与其在体外结合端粒序列的能力一致。在间期核中，再次与抗topoII相反，抗RAP 1给出了一个明显的点状染色，主要位于核周边。在用抗RAP 1抗体染色的二倍体细胞核中可以鉴定出大约16个明亮染色的病灶，这表明端粒可能通过与核膜的相互作用而聚集在一起。
Topoisomerase II (topoII) and RAP1 (Repressor Activator Protein 1) are two abundant nuclear proteins with proposed structural roles in the higher-order organization of chromosomes. Both proteins co-fractionate as components of nuclear scaffolds from vegetatively growing yeast cells, and both proteins are present as components of pachytene chromosome, co-fractionating with an insoluble subfraction of meiotic nuclei. Immunolocalization using antibodies specific for topoII shows staining of an axial core of the yeast meiotic chromosome, extending the length of the synaptonemal complex. RAP1, on the other hand, is located at the ends of the paired bivalent chromosomes, consistent with its ability to bind telomeric sequences in vitro. In interphase nuclei, again in contrast to anti-topoII, anti-RAP1 gives a distinctly punctate staining that is located primarily at the nuclear periphery. Approximately 16 brightly staining foci can be identified in a diploid nucleus stained with anti-RAP1 antibodies, suggesting that telomeres are grouped together, perhaps through interaction with the nuclear envelope.