REGULATION OF CILIARY MOTILITY BY MEMBRANE-POTENTIAL IN PARAMECIUM - A ROLE FOR CYCLIC-AMP
REGULATION OF CILIARY MOTILITY BY MEMBRANE-POTENTIAL IN PARAMECIUM - A ROLE FOR CYCLIC-AMP
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DOI：
10.1002/cm.970060303
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发表时间：
1986-01-01
影响因子：
--
通讯作者：
NELSON, DL
中科院分区：
文献类型：
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作者：
BONINI, NM;GUSTIN, MC;NELSON, DL
草履虫的膜电位控制纤毛跳动的频率和方向，从而决定细胞的游泳行为。使膜电位超极化的刺激会增加纤毛的跳动频率，从而增加向前游泳的速度。我们观察到：1) 提高细胞内环 AMP 的药物使游泳速度增加 2-3 倍，2) 通过操纵细胞外阳离子（例如 K+）使膜电位超极化，与对照相比，诱导环 AMP 的瞬时增加和更高的持续水平，3) 添加环 AMP 后，MgATP 中去污剂透化细胞的游动速度被刺激 2 倍。我们的结果表明，膜电位可以调节草履虫的细胞内 cAMP，并且膜电位对游泳速度的控制可能部分是由 cAMP 介导的。
The membrane potential of Paramecium controls the frequency and direction of the ciliary beat, thus determining the cell''s swimming behavior. Stimuli that hyperpolarize the membrane potential increase the ciliary beat frequency and therefore increase forward swimming speed. We have observed that 1) drugs that elevate intracellular cyclic AMP increased swimming speed 2-3-fold, 2), hyperpolarizing the membrane potential by manipulation of extracellular cations (e.g., K+) induced both a transient increase in, and a higher sustained level of cyclic AMP compared to the control, and 3) the swimming speed of detergent-permeabilized cells in MgATP was stimulated 2-fold by the addition of cyclic AMP. Our results suggest that the membrane potential can regulate intracellular cAMP in Paramecium and that control of swimming speed by membrane potential may in part be mediated by cAMP.