Characterization of RAC3, a novel member of the Rho family
Characterization of RAC3, a novel member of the Rho family
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DOI：
10.1074/jbc.272.33.20384
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发表时间：
1997-08-15
影响因子：
4.8
通讯作者：
Heisterkamp, N
中科院分区：
文献类型：
--
作者：
Haataja, L;Groffen, J;Heisterkamp, N
小 GTP 结合蛋白 Rad 和 Rac2 对于调节真核细胞中的多种信号转导途径至关重要。在此，我们报告了新的第三个 Rac 家族成员 Rac3 的分离。 Rac3 与 Rac1/2 的不同之处在于其羧基末端，这是一个与亚细胞定位和与特定细胞调节因子结合相关的结构域。 RAC3 mRNA 表达模式不同于造血特异性的 RAGS，也不同于 RAC1。 RAC3 基因被定位到染色体 17q23-25，这是乳腺癌中经常缺失的区域。 Rac3 蛋白水平不受肌动蛋白细胞骨架组织的影响，但值得注意的是，是血清诱导的，Rac3 是一种活性 GTP 酶，并且该活性受 Bcr 调节。当组成型激活时，Rac3 能够有效刺激 c-Jun 氨基末端激酶信号传导通路。这些发现支持 Rac3 在细胞内信号传导中的作用。
The small GTP-binding proteins Rad and Rac2 are critically important in regulating multiple signal transduction pathways in eukaryotic cells, Here we report the isolation of a novel third Rac family member, Rac3. Rac3 differs from Rac1/2 at its carboxyl-terminal end, a domain associated with subcellular localization and binding to specific cellular regulators. RAC3 mRNA expression patterns differ from those of RAGS, which is hematopoietic specific and also from those of RAC1. The RAC3 gene was mapped to chromosome 17q23-25, a region frequently deleted in breast cancer. Rac3 protein levels ape not affected by organization of the actin cytoskeleton but remarkably, are serum-inducible, Rac3 is an active GTPase, and this activity is regulated by Bcr. When constitutively activated, Rac3 is able to stimulate efficiently the c-Jun amino-terminal kinase signaling pathway, These findings support a role for Rac3 in intracellular signaling.