Rat hepatic nuclear cytochrome P-450 and epoxide hydrase in membranes prepared by two methods: similarities with the microsomal enzymes.
Rat hepatic nuclear cytochrome P-450 and epoxide hydrase in membranes prepared by two methods: similarities with the microsomal enzymes.
复制标题
大鼠肝核细胞色素P-450和环氧化物水合酶的膜制备方法有两种：与微粒体酶相似。
作者：
Hasan Mukhtar;T. H. Elmamlouk;R. Philpot;J. Bend
未处理、PB预处理或3-MC预处理动物的大鼠肝核膜中的细胞色素P-450在光谱上与相应的肝微粒体相似。PB可诱导肝细胞核膜和微粒体的环氧水合酶活性，3-MC无诱导作用。无论是用DNA酶消化法还是蔗糖密度离心法制备核膜，都得到了相同的结果。
Cytochrome P-450 in rat liver nuclear membranes of untreated, PB-pretreated, or 3-MC-pretreated animals was spectrally similar to corresponding liver microsomes. Epoxide hydrase activity of liver nuclear membrane and microsomes was induced by PB administration; 3-MC did not cause any induction. The same results were obtained when either the DNase digestion method or sucrose density centrifugation technique for preparation of nuclear membranes was used.