A novel method for transformation of intact yeast cells by electroinjection of plasmid DNA
A novel method for transformation of intact yeast cells by electroinjection of plasmid DNA
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一种通过电注射质粒 DNA 转化完整酵母细胞的新方法
DOI：
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发表时间：
1985
影响因子：
5
通讯作者：
A. Kimura
中科院分区：
文献类型：
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作者：
Hideaki Hashimoto;H. Morikawa;Yasuyuki Yamada;A. Kimura
发现质粒DNA（YEp 13）可以通过电场脉冲导入完整的酵母细胞中。通过这种电注入方法的变换频率取决于初始电场强度、放电电容器的电容和施加的脉冲的数量。在初始强度为5 KV/cm、电容为1 μF的条件下，连续三次脉冲可获得最大数量的转化体（90±20个/μgDNA）。本发明的电注射方法简单，并且转化处理后2至3天内可获得转化体。
SummaryIt was found that plasmid DNA (YEp13) can be introduced into intact yeast cells by electric field pulses. The frequency of transformation by this electroinjection method depend upon the initial electric field intensity, the capacitance of the electric discharge capacitor, and the number of pulses applied. A maximum number of transformants (90±20/μgDNA) was obtained by three successive pulses with an initial intensity of 5 KV/cm and with a capacitance of 1 μF. The present electroinjection method is simple, and transformants can be obtained within 2 to 3 days after transformation treatment.