Myasthenia induced by monoclonal anti-acetylcholine receptor antibodies: clinical and electrophysiological aspects.
Myasthenia induced by monoclonal anti-acetylcholine receptor antibodies: clinical and electrophysiological aspects.
复制标题
单克隆抗乙酰胆碱受体抗体诱导的肌无力：临床和电生理方面。
DOI：
10.1002/ana.410090609
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发表时间：
1981
影响因子：
11.2
通讯作者：
Richman,DP
中科院分区：
文献类型：
--
作者：
Burres,SA;Crayton,JW;Gomez,CM;Richman,DP
为探讨实验性肌无力中抗乙酰胆碱受体的体液免疫反应的各个组成部分，我们将抗乙酰胆碱受体的单克隆抗体注射到正常大鼠体内。这些抗体由免疫脾细胞与骨髓瘤细胞系P3-X63-Ag 8融合形成的克隆杂交瘤细胞系产生。来自7个克隆中的2个克隆的抗体诱导急性实验性肌无力，表现为临床虚弱、对重复神经刺激的肌电反应下降以及微型终板电位（mepps）的平均振幅降低：0.55 ± 0.02 mv（SEM）与对照组的0.71 ± 0.02 mv（p< 0.001）。在临床受影响的动物中，膈肌的mepp振幅与腓肠肌的递减反应相关（r=-0.91，p < 0.01）。结果表明，单一抗体种类与乙酰胆碱受体分子上的单一决定簇的结合足以诱导急性实验性重症肌无力。
To investigate individual components of the humoral immune response directed against the acetylcholine receptor in experimental myasthenia, we injected monoclonal antireceptor antibody into normal rats. These antibodies were produced by cloned hybridoma lines formed by fusion of immune spleen cells with the myeloma cell line P3‐X63‐Ag8. Antibodies from two of seven clones induced acute experimental myasthenia, manifested by clinical weakness, decremental electromyographic response to repetitive nerve stimulation, and diminished mean amplitudes of miniature end‐plate potentials (mepps): 0.55 ± 0.02 mv (SEM) versus 0.71 ± 0.02 mv for controls (p< 0.001). In clinically affected animals, mepp amplitudes from the diaphragm correlated with decremental responses in the gastrocnemius muscle (r= −0.91,p< 0.01). The results suggest that binding of a single antibody species to a single determinant on the acetylcholine receptor molecule is sufficient to induce acute experimental myasthenia gravis.