Facile synthesis of 5 nm NaYF4:Yb/Er nanoparticles for targeted upconversion imaging of cancer cells
Facile synthesis of 5 nm NaYF4:Yb/Er nanoparticles for targeted upconversion imaging of cancer cells
复制标题
轻松合成 5 nm NaYF4:Yb/Er 纳米粒子，用于癌细胞的靶向上转换成像
DOI：
10.1016/j.talanta.2016.02.039
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发表时间：
2016-05-15
期刊：
影响因子：
6.1
通讯作者：
Zhao, Xiaojun
中科院分区：
文献类型：
--
作者：
Hu, Yueli;Wu, Boyue;Zhao, Xiaojun
首次采用简单的“绿色”策略合成了具有纯β相的5 nm强绿色发射的NaYF4:Yb/Er上转换纳米粒子（UCNPs）。传统的有机溶剂热法通常用于制备高质量、均匀的统一纳米粒子，但油酸镧配合物的制备过程繁琐，需要加热和多步后处理进行纯化。水醇溶剂热法对环境友好，但制备的UCNPs尺寸大、生物相容性差、细胞毒性高，限制了其在细胞成像中的应用。本文以稀土硝酸盐RE(NO3)(3) （RE = Y-0.80 Yb-0.18 Er-0.02）为前驱体，二甘醇(DEG)/乙二醇(EG)/水为溶剂制备了NaYF4:Yb/Er UCNPs。提出了一种温度控制在300℃的绿色溶剂热法。对制备的NaYF4:Yb/Er UCNPs进行了表征，发现其UC发射增强，粒径可控。制备的UCNPs通过叶酸涂层进一步功能化，用于靶向成像和提高生物相容性。强的上转换发光、优异的生物相容性和超小的尺寸使UCNPs具有上转换生物成像活细胞的潜力。所制备的UCNPs具有良好的胶体稳定性和较低的细胞毒性，以及所开发的合成方案可能推动未来UCNPs和基于生物分子的纳米技术的研究。(C) 2016 Elsevier B.V.版权所有
5 nm intense green emission NaYF4:Yb/Er upconversion nanoparticles (UCNPs) with pure beta phase was synthesized with a simple "green" strategy for the first time. Traditional organic solvothermal method is often applied to prepare the high-quality and uniform UCNPs, but the preparation of lanthanide-oleate complexes is laborious as heating and multistep post-treatment for purification are often required. The water-alcohols solvothermal method is environmentally friendly, but the fabricated UCNPs have big size, poor biocompatibility and high cytotoxicity, which limited their application for cell imaging. Herein, NaYF4:Yb/Er UCNPs were prepared with rare-earth nitrates RE(NO3)(3) (RE = Y-0.80 Yb-0.18 Er-0.02) as precursors and diethylene glycol (DEG)/ethylene glycol (EG)/water as the solvent. A facile green solvothermal method with the temperature being controlled at 300 degrees C was developed. The as-prepared NaYF4:Yb/Er UCNPs were characterized and were found to have enhanced UC emission and controllable particle size. The as-prepared UCNPs were further functionalized via folic acid coating for the targeted imaging and improved biocompatibility. It was made the UCNPs potential for upconversion bioimaging of living cells by the strong upconversion luminescence, the excellent biocompatibility, and the super-small size. The good colloidal stability and low cell cytotoxicity of the as-prepared UCNPs and the developed synthesis protocol might advance both the fields of UCNPs and biomolecule-based nanotechnology for future studies. (C) 2016 Elsevier B.V. All rights reserved.