WRKY1 Mediates Transcriptional Regulation of Light and Nitrogen Signaling Pathways
WRKY1 Mediates Transcriptional Regulation of Light and Nitrogen Signaling Pathways
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DOI：
10.1104/pp.19.00685
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发表时间：
2019-11-01
期刊：
影响因子：
7.4
通讯作者：
Marshall-Colon, Amy
中科院分区：
文献类型：
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作者：
Heerah, Sachin;Katari, Manpreet;Marshall-Colon, Amy
植物对多种环境刺激的反应必须被整合，以使它们能够适应它们的代谢和发育。光和氮（N）是两种这样的刺激，其下游信号传导途径必须彼此密切连接以控制植物能量状态。在这里，我们描述的WRKY1转录因子在控制拟南芥（拟南芥）叶的全基因组转录重编程响应个人和组合的光和N信号的功能作用。这包括由WRKY1调控的724个基因组成的交叉调控网络，并参与N和光信号通路。WRKY1基因功能的丧失对参与氮吸收和同化（初级代谢）以及胁迫响应途径（次级代谢）的基因的光和氮响应具有显著影响。我们在转录组和代谢物分析水平的结果支持一个模型，其中WRKY1使植物能够激活参与细胞碳资源回收的基因，当光限制，但氮是丰富的，上调氨基酸代谢时，光和氮都限制。在这种潜在的能量守恒机制中，WRKY1整合了细胞氮和光能资源的信息，从而引发植物代谢的变化。
Plant responses to multiple environmental stimuli must be integrated to enable them to adapt their metabolism and development. Light and nitrogen (N) are two such stimuli whose downstream signaling pathways must be intimately connected to each other to control plant energy status. Here, we describe the functional role of the WRKY1 transcription factor in controlling genome-wide transcriptional reprogramming of Arabidopsis (Arabidopsis thaliana) leaves in response to individual and combined light and N signals. This includes a cross-regulatory network consisting of 724 genes regulated by WRKY1 and involved in both N and light signaling pathways. The loss of WRKY1 gene function has marked effects on the light and N response of genes involved in N uptake and assimilation (primary metabolism) as well as stress response pathways (secondary metabolism). Our results at the transcriptome and at the metabolite analysis level support a model in which WRKY1 enables plants to activate genes involved in the recycling of cellular carbon resources when light is limiting but N is abundant and upregulate amino acid metabolism when both light and N are limiting. In this potential energy conservation mechanism, WRKY1 integrates information about cellular N and light energy resources to trigger changes in plant metabolism.