Role of the rab5 guanine nucleotide exchange factor, Rme-6, in the regulation of clathrin-coated vesicle uncoating.
Role of the rab5 guanine nucleotide exchange factor, Rme-6, in the regulation of clathrin-coated vesicle uncoating.
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DOI：
10.1007/978-1-4939-2569-8_24
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发表时间：
2015
影响因子：
--
通讯作者：
E. Smythe
中科院分区：
文献类型：
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作者：
E. Smythe
网格蛋白包被的小凹是进入真核细胞的主要端口。在网格蛋白包被的小坑分裂形成包被的囊泡后，需要去除形成包被的外周膜蛋白。在这里，我们描述了一种测定完整细胞中网格蛋白涂层脱壳程度的测定方法。该测定已用于研究 rab5 依赖性脱衣调节。具体来说，它已被用于识别 rab5 鸟嘌呤核苷酸交换因子之间的功能差异。
Clathrin-coated pits are major ports of entry into eukaryotic cells. Following scission of clathrin-coated pits to form coated vesicles, the peripheral membrane proteins that form the coat need to be removed. Here we describe an assay that provides a measure of the extent of clathrin coat uncoating in intact cells. This assay has been used to investigate rab5-dependent modulation of uncoating. Specifically, it has been used to identify functional differences between rab5 guanine nucleotide exchange factors.