Pulsed Electromagnetic Fields Increase Angiogenesis and Improve Cardiac Function After Myocardial Ischemia in Mice
Pulsed Electromagnetic Fields Increase Angiogenesis and Improve Cardiac Function After Myocardial Ischemia in Mice
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脉冲电磁场可增加小鼠心肌缺血后的血管生成并改善心脏功能
DOI：
10.1253/circj.cj-19-0758
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发表时间：
2020-02-01
影响因子：
3.3
通讯作者：
Wei, Quan
中科院分区：
文献类型：
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作者：
Peng, Lihong;Fu, Chenying;Wei, Quan
背景：以往的研究表明，脉冲电磁场（PEMF）刺激血管生成，可能是改善心肌梗死（MI）后心功能的潜在治疗策略。本研究探讨了PEMF对心肌梗死小鼠的作用及其相关机制。方法和结果：使用MI小鼠进行PEMF治疗（15 Hz 1.5 mT PEMF或30 Hz 3.0 mT PEMF），每天45分钟，持续2周。此外，采用体内基质塞法观察PEMF促进血管生成的作用。与假PEMF组比较，30 Hz 3.0 mT的PEMF治疗可显著改善心功能。15 Hz 1.5 mT和30 Hz 3.0 mT的PEMF处理均增加了毛细血管密度，缩小了梗死面积，增加了血管内皮生长因子（VEGF）、血管内皮生长因子受体2 （VEGFR2）、Ser473磷酸化Akt （p(Ser473)-Akt）和S1177磷酸化内皮一氧化氮合酶（p(S1177)-eNOS）的蛋白表达，增加了梗死边界区VEGF和缺氧诱导因子1- α (HIF-1 α)的mRNA水平。此外，30 Hz 3.0 mT处理还能提高成纤维细胞生长因子2 （FGF2）蛋白和mRNA水平以及β 1整合素蛋白水平，显示出更强的治疗效果。结论：PEMF治疗可促进心肌梗死边缘区血管生成，改善心功能。30 Hz 3.0 mT的治疗参数对心肌梗死小鼠显著有效。这种作用与HIF-1 α /VEGF/Akt/eNOS或HIF-1 α /FGF2/Akt/eNOS的促血管生成信号通路有关。
Background: Previous studies have shown that pulsed electromagnetic fields (PEMF) stimulate angiogenesis and may be a potential treatment strategy to improve cardiac function after myocardial infarction (MI). This study explored the effects and its related mechanisms of PEMF in MI mice.Methods and Results: MI mice were used in PEMF treatment (15 Hz 1.5 mT PEMF or 30 Hz 3.0 mT PEMF) for 45 min per day for 2 weeks. Furthermore, an in vivo Matrigel plug assay was used to observe the effect of PEMF in promoting angiogenesis. Compared with the sham PEMF group, PEMF treatment with 30 Hz 3.0 mT significantly improved heart function. PEMF treatment with 15 Hz 1.5 mT and 30 Hz 3.0 mT both increased capillary density, decreased infarction area size, increased the protein expression of vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor 2 (VEGFR2), Ser473-phosphorylated Akt (p(Ser473)-Akt) and S1177-phosphorylated endothelial nitric oxide synthase (p(S1177)-eNOS), and increased the mRNA level of VEGF and hypoxia inducible factor 1-alpha (HIF-1 alpha) in the infarct border zone. Additionally, treatment with 30 Hz 3.0 mT also increased protein and mRNA level of fibroblast growth factor 2 (FGF2), and protein level of beta 1 integrin, and shows a stronger therapeutic effect.Conclusions: PEMF treatment could promote angiogenesis of the infarct border zone and improve cardiac function in MI mice. A treatment parameter of 30 Hz 3.0 mT is remarkably effective in MI mice. The effect is associated with the proangiogenic signaling pathways of HIF-1 alpha/VEGF/Akt/eNOS or HIF-1 alpha/FGF2/Akt/eNOS.