The Escherichia coli RNA processing and degradation machinery is compartmentalized within an organized cellular network.
The Escherichia coli RNA processing and degradation machinery is compartmentalized within an organized cellular network.
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DOI：
10.1042/bj20131287
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发表时间：
2014-02
期刊：
影响因子：
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通讯作者：
A. Taghbalout;Qingfen Yang;V. Arluison
中科院分区：
文献类型：
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作者：
A. Taghbalout;Qingfen Yang;V. Arluison
细菌RNA加工和降解涉及大量RNA酶、RNA解旋酶和其他蛋白质的协调作用。目前尚不清楚该功能网络在细胞内是如何组织的，也不知道它是如何协调或调节的。在本研究中，我们表明，大肠杆菌 RNA 降解和加工网络的多个成分位于似乎盘绕在细胞外围的扩展细胞结构内。除了之前报道的蛋白质 RNase II 和 RNaseE 之外，还包括 Orn、Hfq、PAP I、RNase III、RppH、RraA 和 RraB。对几种蛋白质的双标记定位研究显示蛋白质在观察到的结构内共定位。蛋白质组装成结构独立于 MreBCD 或 MinCDE 细胞骨架系统、RNA 合成或细胞内的类核定位。我们的结果表明，RNA 加工和降解网络的组成部分在细胞内划分，而不是广泛分布在细胞质中。这种隔离为细胞提供了一种可能的机制来控制对 RNA 底物的获取，并在功能上协调 RNA 加工和降解途径的多个参与者。
Bacterial RNA processing and degradation involves the co-ordinated action of a large number of RNases, RNA helicases and other proteins. It is not known how this functional network is organized within the cell nor how it is co-ordinated or regulated. In the present study, we show that multiple components of the RNA degradation and processing network of Escherichia coli are localized within extended cellular structures that appear to coil around the periphery of the cell. These include Orn, Hfq, PAP I, RNase III, RppH, RraA and RraB in addition to the previously reported proteins RNase II and RNaseE. Double-label localization studies of several of the proteins showed co-localization of the proteins within the observed structures. Assembly of the proteins into the structures was independent of the MreBCD or MinCDE cytoskeletal systems, RNA synthesis, or nucleoid positioning within the cell. Our results indicate that the components of the RNA processing and degradation network are compartmentalized within the cell rather than diffusely distributed in the cytoplasm. This sequestration provides the cell with a possible mechanism to control access to RNA substrates and to functionally co-ordinate the multiple players of the RNA processing and degradation pathways.