No evidence for the presence of oogonia in the human ovary after their final clearance during the first two years of life
No evidence for the presence of oogonia in the human ovary after their final clearance during the first two years of life
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DOI：
10.1093/humrep/der145
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发表时间：
2011-08-01
影响因子：
6.1
通讯作者：
Mollgard, K.
中科院分区：
文献类型：
--
作者：
Byskov, A. G.;Hoyer, P. E.;Mollgard, K.
背景技术背景：对小鼠和人类的研究结果证实或否定了卵原细胞/原始生殖细胞在出生后卵巢中的存在。本研究的目的是跟踪是否卵原细胞识别的免疫组化方法在第一个三个月的人类卵巢也存在于围产期和产后oars.METHODS：对于这项研究，82人卵巢收集：25从胚胎5至10周后的概念（wpc），2在18 wpc，32从32 wpc到2年，23从2到32年。其中，80个卵巢被固定并石蜡包埋，2个（8岁）卵巢被处理用于塑料切片。制备连续切片用于卵原细胞标记物的免疫组织化学检测：干细胞因子酪氨酸激酶受体（SCF）（C-KIT）、阶段特异性胚胎抗原-4（SSEA 4）、同源框基因转录因子（NANOG）、八聚体结合转录因子4（OCT 4）和黑素瘤抗原-4（Mage-A4），同时注意到C-KIT也染色双线期卵母细胞。几乎所有的卵原细胞都只对SSEA 4、NANOG、OCT 4和C-KIT染色，而MAGE-A4仅对一小部分染色。出生时只有少数卵原细胞染色。这些在2年前消失，只留下双线期卵母细胞C-KIT染色。从18周胎龄到2岁，髓质包含健康和退化的卵原细胞和小卵泡，废物篮（WB）和卵原细胞封闭在生长卵泡（FWB）中的聚集体。老年人卵巢髓质包含原始的，健康的follicles.CONCLUSIONS：我们发现没有证据证明卵原细胞在人类卵巢的存在后，他们最终清除在第一个2年。我们认为，围产期髓质WB和FWB引起的髓质中的小，健康的卵泡群。
BACKGROUND: Conflicting results of studies on mouse and human have either verified or refuted the presence of oogonia/primordial germ cells in the post-natal ovary. The aim of this study was to trace whether oogonia recognized by immunohistochemical methods in the first trimester human ovary were present also in peri-and post-natal ovaries.METHODS: For this study, 82 human ovaries were collected: 25 from embryos from 5 to 10 weeks post conception (wpc), 2 at 18 wpc, 32 from 32 wpc to 2 years and 23 from 2 to 32 years. Of these, 80 ovaries were fixed and paraffin-embedded and 2 (8 year-old) ovaries were processed for plastic sections. Serial sections were prepared for immunohistochemical detection of markers for oogonia: tyrosine kinase receptor for stem cell factor (SCF)(C-KIT), stage-specific embryonic antigen-4 (SSEA4), homeobox gene transcription factor (NANOG), octamer binding transcription factor 4 (OCT4) and melanoma antigen-4 (Mage-A4), while noting that C-KIT also stains diplotene oocytes.RESULTS: Almost all oogonia exclusively stained for SSEA4, NANOG, OCT4 and C-KIT, whereas MAGE-A4 only stained a small fraction. At birth only a few oogonia were stained. These disappeared before 2 years, leaving only diplotene oocytes stained for C-KIT. From 18 wpc to 2 years, the medulla contained conglomerates of healthy and degenerating oogonia and small follicles, waste baskets (WBs) and oogonia enclosed in growing follicles (FWB). Medulla of older ovaries contained groups of primordial, healthy follicles.CONCLUSIONS: We found no evidence for the presence of oogonia in the human ovary after their final clearing during the first 2 years. We suggest that perinatal medullary WB and FWB give rise to the groups of small, healthy follicles in the medulla.