Ectopic Pax2 expression in chick ventral optic cup phenocopies loss of Pax2 expression.
Ectopic Pax2 expression in chick ventral optic cup phenocopies loss of Pax2 expression.
复制标题
鸡腹侧视杯表型中的异位 Pax2 表达丧失了 Pax2 表达。
DOI：
10.1016/j.ydbio.2008.03.041
复制
发表时间：
2008
影响因子：
2.7
通讯作者：
Belecky-Adams,TeriL
中科院分区：
文献类型：
--
作者：
Sehgal,Rachna;Karcavich,Rachel;Carlson,Scott;Belecky-Adams,TeriL
Pax2对于泌尿生殖系统、神经管、耳囊、视杯和视束的发育是必不可少的[Dressler，G.R.，Deutsch，U，et al.，1990。一种新的小鼠成对框基因Pax2及其在发育中的排泄系统中的表达发展109(4)，787-795；Nornes，H.O.，Dressler，G.R.等人，1990年。Pax2在小鼠神经发生过程中的时空限制性表达。发展109(4)，797-809；埃克尔斯，M.R.，沃利斯，L.J.等人，1992年。PAX2基因在人胎肾和肾母细胞瘤中的表达细胞生长差异3(5)，279-289]。在视觉系统中，功能丧失导致脉络膜裂隙不闭合(称为缺损区)，视神经星形胶质细胞丢失，以及视交叉处异常的轴突寻径[Probine，J.，Sandulache，R.，等，1996]。小鼠Pax2(1Neu)突变与人类PAX2突变相同，该突变发生在一个患有肾缺损综合征的家庭中，并导致大脑、耳朵、眼睛和肾脏的发育缺陷。程序娜塔莉。阿卡德。SCI。《美国判例汇编》93(24)，13870-13875；托雷斯，M.，戈麦斯-帕尔多，E.等人，1996年。Pax2有助于内耳模式和视神经轨迹。发展122(11)、3381-3391]。本研究旨在确定在发现Pax2的正常发育期后，Pax2在鸡腹侧视杯中的异位表达的影响。在卵子中，将Pax2电穿孔到鸡的腹侧视杯中会导致缺损的形成，这种情况通常与Pax2表达的缺失有关。虽然Pax2的过度表达表现为Pax2的丢失，但脉络膜裂隙闭合失败的机制与细胞命运从视网膜腹侧和视网膜色素上皮(RPE)转变为星形胶质细胞的命运有关。此外，Pax2在RPE中的异位表达似乎对相邻的RPE具有非细胞自主效应，产生了替代RPE的异位神经视网膜。
Pax2 is essential for the development of the urogenital system, neural tube, otic vesicle, optic cup and optic tract [Dressler, G.R., Deutsch, U., et al., 1990. PAX2, a new murine paired-box-containing gene and its expression in the developing excretory system. Development 109 (4), 787–795; Nornes, H.O., Dressler, G.R., et al., 1990. Spatially and temporally restricted expression of Pax2 during murine neurogenesis. Development 109 (4), 797–809; Eccles, M.R., Wallis, L.J., et al., 1992. Expression of the PAX2 gene in human fetal kidney and Wilms’ tumor. Cell Growth Differ 3 (5), 279–289]. Within the visual system, a loss-of-function leads to lack of choroid fissure closure (known as a coloboma), a loss of optic nerve astrocytes, and anomalous axonal pathfinding at the optic chiasm [Favor, J., Sandulache, R., et al., 1996. The mouse Pax2(1Neu) mutation is identical to a human PAX2 mutation in a family with renal-coloboma syndrome and results in developmental defects of the brain, ear, eye, and kidney. Proc. Natl. Acad. Sci. U. S. A. 93 (24), 13870–13875; Torres, M., Gomez-Pardo, E., et al., 1996. Pax2 contributes to inner ear patterning and optic nerve trajectory. Development 122 (11), 3381–3391]. This study is directed at determining the effects of ectopic Pax2 expression in the chick ventral optic cup past the normal developmental period when Pax2 is found. In ovo electroporation of Pax2 into the chick ventral optic cup results in the formation of colobomas, a condition typically associated with a loss of Pax2 expression. While the overexpression of Pax2 appears to phenocopy a loss of Pax2, the mechanism of the failure of choroid fissure closure is associated with a cell fate switch from ventral retina and retinal pigmented epithelium (RPE) to an astrocyte fate. Further, ectopic expression of Pax2 in RPE appears to have non-cell autonomous effects on adjacent RPE, creating an ectopic neural retina in place of the RPE.