The chemical component of the mixed GF-TTMn synapse in Drosophila melanogaster uses acetylcholine as its neurotransmitter.
The chemical component of the mixed GF-TTMn synapse in Drosophila melanogaster uses acetylcholine as its neurotransmitter.
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DOI：
10.1111/j.1460-9568.2007.05686.x
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发表时间：
2007-07
期刊：
影响因子：
--
通讯作者：
Murphey RK
中科院分区：
文献类型：
--
作者：
Allen MJ;Murphey RK
成年果蝇最大的中央突触是一个混合的电化学突触，其间隙连接需要shaking-B（shak-B）基因的产物。Shak-B2突变果蝇缺乏间隙连接在这个突触，这是之间的巨纤维（GF）和tergotrophonteral运动神经元（TTMn），但它仍然表现出一个长的潜伏期响应GF刺激。我们在shak-B2果蝇中将破伤风毒素轻链的表达靶向于GF，以阻断化学传播。在tergotrophonteral肌肉（TTM）的长潜伏期反应被取消，表明突触的化学成分介导这种反应。通过cha-GAL 80转基因减弱GAL 4介导的标记，揭示了GF是胆碱能的。我们已经使用了一个温度敏感的等位基因的胆碱乙酰转移酶基因（chats 2），以阻止胆碱能突触在成年苍蝇，这也取消了长潜伏期的反应，在shak-B2苍蝇。总之，数据提供的证据表明，这两个组件的混合突触的功能和之间的GF和TTMn的化学神经递质是乙酰胆碱。我们的研究结果表明，这种突触的两个组成部分可以分开，以便进一步研究混合突触的建立和功能的机制。
The largest central synapse in adult Drosophila is a mixed electro-chemical synapse whose gap junctions require the product of the shaking-B (shak-B) gene. Shak-B2 mutant flies lack gap junctions at this synapse, which is between the giant fibre (GF) and the tergotrochanteral motor neuron (TTMn), but it still exhibits a long latency response upon GF stimulation. We have targeted the expression of the light chain of tetanus toxin to the GF, to block chemical transmission, in shak-B2 flies. The long latency response in the tergotrochanteral muscle (TTM) was abolished indicating that the chemical component of the synapse mediates this response. Attenuation of GAL4-mediated labelling by a cha-GAL80 transgene, reveals the GF to be cholinergic. We have used a temperature-sensitive allele of the choline acetyltransferase gene (chats2) to block cholinergic synapses in adult flies and this also abolished the long latency response in shak-B2 flies. Taken together the data provide evidence that both components of this mixed synapse are functional and that the chemical neurotransmitter between the GF and the TTMn is acetylcholine. Our findings show that the two components of this synapse can be separated to allow further studies into the mechanisms by which mixed synapses are built and function.
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影响因子：
9.8
作者：
Fayyazuddin A;Zaheer MA;Hiesinger PR;Bellen HJ
通讯作者：
Bellen HJ
影响因子：
3.5
作者：
Allen, MJ;Shan, XL;Murphey, RK
通讯作者：
Murphey, RK
影响因子：
25
作者：
Godenschwege, TA;Hu, HL;Murphey, RK
通讯作者：
Murphey, RK
DOI：
10.1007/s00359-006-0203-9
发表时间：
2007-04-01
影响因子：
2.1
作者：
Hammond, Sarah;O'Shea, Michael
通讯作者：
O'Shea, Michael
影响因子：
64.8
作者：
Phelan, P;Stebbings, LA;Ford, C
通讯作者：
Ford, C