Effects of low-intensity prolonged exercise on PGC-1 mRNA expression in rat epitrochlearis muscle
Effects of low-intensity prolonged exercise on PGC-1 mRNA expression in rat epitrochlearis muscle
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DOI：
10.1016/s0006-291x(02)00881-1
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发表时间：
2002-08-16
影响因子：
3.1
通讯作者：
Tabata, I
中科院分区：
文献类型：
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作者：
Terada, S;Goto, M;Tabata, I
我们曾报道过游泳运动训练后大鼠上滑车肌过氧化物酶体增殖物激活受体γ共激活因子-1（PGC-1）mRNA表达增加。在本研究中。我们进一步证实，在4-5周龄雄性Sprague-Dawley大鼠的上滑车肌中，PGC-1 mRNA表达在6小时急性低强度游泳运动后增加。随着这种增加，表达水平大约是对照组和浸泡组大鼠的8倍，浸泡组大鼠在温水中停留6小时，保持在与游泳桶相同的温度（35摄氏度）（p < 0.01）。一下PGC-1 mRNA在肌肉中的表达被发现有增加6小时后30 10秒强直收缩，在体外电刺激诱导。终于来了用0.5mM 5-氨基咪唑-4-甲酰胺核糖核苷（AICAR：一种5 ′ AMP-活化的蛋白激酶（AMPK）激活剂）孵育18小时的肌肉中的PGC-1 mRNA表达升高至对照肌肉的约3倍（n = 6，p < 0.001）。还发现游泳后滑车上肌中AMPK活性升高至运动前值的约4倍（p < 0.001）。这些结果可能。表明急性低强度长时间游泳运动直接增强运动过程中激活肌肉中PGC-1 mRNA的表达，可能至少部分通过AMPK相关机制。(C)2002 Elsevier Science（美国）。All rights reserved.
We previously reported that the peroxisome proliferator-activated receptor gamma coactivator-1 (PGC-1) mRNA in rat epitrochlearis muscle was increased after swimming exercise training. In the present study. we demonstrated further that PGC-1 mRNA expression in the epitrochlearis muscle of 4-5-week-old male Sprague-Dawley, rats was increased after a 6-h acute bout of low-intensity swimming exercise. With this increase, the expression level was approximately 8-fold of control and immersion group rats that stayed for 6-h in warm water, maintained at the identical temperature of the swimming barrel (35degreesC) (p < 0.01). Second. PGC-1 mRNA expression in the muscle was found to have increased 6-h after 30 10-s tetani contractions were induced by, in vitro electrical stimulation. Finally. PGC-1 mRNA expression in the muscle incubated for 18-h with 0.5 mM 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR: a 5' AMP-activated protein kinase (AMPK) activator) was elevated to approximately 3-fold of the control muscle (n = 6 p < 0.001). AMPK activity in epitrochlearis muscle after the swimming was also found to be elevated to approximately 4-fold of the pre-exercise value (p < 0.001). These results may. suggest that an acute bout of low-intensity prolonged swimming exercise directly enhances the PGC-1 mRNA expression in the activated muscle during exercise, possibly through, at least in part, an AMPK-related mechanism. (C) 2002 Elsevier Science (USA). All rights reserved.