Quantitation of the flavonoid wogonin and its major metabolite wogonin-7β-D-glucuronide in rat plasma by liquid chromatographytandem mass spectrometry
Quantitation of the flavonoid wogonin and its major metabolite wogonin-7β-D-glucuronide in rat plasma by liquid chromatographytandem mass spectrometry
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DOI：
10.1016/s1570-0232(02)00288-x
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发表时间：
2002-08-05
影响因子：
3
通讯作者：
Zhong, DF
中科院分区：
文献类型：
--
作者：
Chen, XY;Wang, HY;Zhong, DF
本研究采用液相色谱-串联质谱法测定大鼠血浆中枸杞素及其主要代谢物的含量。通过色谱和电喷雾多级质谱分析，只鉴定出一种与葡萄糖醛酸结合的代谢物。与2-氯乙醇的衍生化反应进一步证明代谢物是w7 -g，而不是w7 -g。其他缀合代谢物，如硫酸盐和糖苷，未检测到。在选定的反应监测模式下，采用常压化学电离源测定血浆游离沃戈宁浓度。该方法对沃戈宁的定量下限为0.25 ng/ml，与现有方法相比，具有更高的灵敏度、选择性和分析速度。血浆样品经β -葡萄糖醛酸酶孵育后可定量测定W-7-G。该定量方法成功应用于大鼠口服5 mg/kg沃戈宁后沃戈宁及其主要代谢物W-7-G的临床前药代动力学研究。(C) 2002 Elsevier Science B.V.版权所有
This study described the application of liquid chromatography-tandem mass spectrometry for the quantitation of wogonin and its major metabolite in rat plasma. Only one conjugated metabolite with glucuronic acid was identified by chromatographic and electrospray multi-stage mass spectrometric assay. A derivatization reaction with 2-chlorethanol further demonstrated that the metabolite was wogonin-7beta-D-glucuronide (W-7-G), not wogonin-5beta-D-glucuronide. Other conjugated metabolites, e.g., sulfates and glucosides, were not detected. The plasma concentration of free wogonin was determined using atmospheric pressure chemical ionization source in the selected reaction monitoring mode. The method had a lower limit of quantitation of 0.25 ng/ml for wogonin, which offered increased sensitivity, selectivity and speed of analysis over an existing method. Incubation of the plasma samples with beta-glucuronidase allows the quantitation of W-7-G. This quantitation method was successfully applied to a preclinical pharmacokinetic study of wogonin and its major metabolite, W-7-G, after an oral administration of 5 mg/kg wogonin to rats. (C) 2002 Elsevier Science B.V. All rights reserved.