Yeast homolog of a cancer-testis antigen defines a new transcription complex
Yeast homolog of a cancer-testis antigen defines a new transcription complex
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DOI：
10.1038/sj.emboj.7601235
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发表时间：
2006-08-09
期刊：
影响因子：
11.4
通讯作者：
Libri, Domenico
中科院分区：
文献类型：
--
作者：
Kisseleva-Romanova, Elena;Lopreiato, Raffaele;Libri, Domenico
我们已经分离出一个新的酵母基因（PCC1），编码一个与人类癌症睾丸抗原同源的因子。我们提供的证据表明，Pcc1p是一种新的转录因子，它的突变影响几个基因的表达，其中一些涉及细胞周期的进展和极化生长。Pcc1p的突变还通过损害佐贺和介体共激活物的募集来影响GAL基因的表达。我们表征了一种新的复合物，它包含Pcc1p，一种激酶，Bud32p，一种推定的内肽酶，Kae1p和两个额外的蛋白质编码的ORF YJL184w和YMLO36w。这些蛋白质之间的遗传和物理相互作用强烈表明，这种复合物是一个功能单位。染色质免疫沉淀实验和pcc 1突变体与转录器和染色质修饰酶突变体的多种遗传相互作用强调了复合物在转录中的直接作用。功能互补实验表明，这组基因的转录功能在整个进化过程中是保守的。
We have isolated a new yeast gene (PCC1) that codes for a factor homologous to human cancer-testis antigens. We provide evidence that Pcc1p is a new transcription factor and that its mutation affects expression of several genes, some of which are involved in cell cycle progression and polarized growth. Mutation of Pcc1p also affects the expression of GAL genes by impairing the recruitment of the SAGA and Mediator co-activators. We characterize a new complex that contains Pcc1p, a kinase, Bud32p, a putative endopeptidase, Kae1p and two additional proteins encoded by ORFs YJL184w and YMLO36w. Genetic and physical interactions among these proteins strongly suggest that this complex is a functional unit. Chromatin immunoprecipitation experiments and multiple genetic interactions of pcc1 mutants with mutants of the transcription apparatus and chromatin modifying enzymes underscore the direct role of the complex in transcription. Functional complementation experiments indicate that the transcriptional function of this set of genes is conserved throughout evolution.