MELATONIN IN EDIBLE PLANTS IDENTIFIED BY RADIOIMMUNOASSAY AND BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY
MELATONIN IN EDIBLE PLANTS IDENTIFIED BY RADIOIMMUNOASSAY AND BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY
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DOI：
10.1111/j.1600-079x.1995.tb00136.x
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发表时间：
1995-01-01
影响因子：
10.3
通讯作者：
SCHLOOT, W
中科院分区：
文献类型：
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作者：
DUBBELS, R;REITER, RJ;SCHLOOT, W
褪黑素是脊椎动物松果腺的主要激素，在动物界广泛分布。它具有多种功能，包括同步昼夜节律和周年节律、刺激免疫功能、可能延长寿命、在体外抑制癌细胞生长以及在体内抑制癌症进展和促进，并且最近被证明是一种有效的羟自由基清除剂和抗氧化剂。羟自由基是氧代谢的高毒性副产物，会损害细胞DNA和其他大分子。在此我们报道，在多种植物中也发现了不同浓度的褪黑素。通过放射免疫测定法对9种不同植物进行测量，褪黑素浓度范围为每毫克蛋白质含0到862皮克褪黑素。通过气相色谱/质谱法验证了褪黑素的存在。我们的研究结果表明，食用含有高水平褪黑素的植物材料可能会改变血液中这种吲哚的褪黑素水平，并为大分子提供抗氧化损伤的保护。
Melatonin, the chief hormone of the pineal gland in vertebrates, is widely distributed in the animal kingdom. Among many functions, melatonin synchronizes circadian and circannual rhythms, stimulates immune function, may increase life span, inhibits growth of cancer cells in vitro and cancer progression and promotion in vivo, and was recently shown to be a potent hydroxyl radical scavenger and antioxidant. Hydroxyl radicals are highly toxic by-products of oxygen metabolism that damage cellular DNA and other macromolecules. Herein we report that melatonin, in varying concentrations, is also found in a variety of plants. Melatonin concentrations, measured in nine different plants by radioimmunoassay, ranged from 0 to 862 pg melatonin/mg protein. The presence of melatonin was verified by gas chromatography/mass spectrometry. Our findings suggest that the consumption of plant materials that contain high levels of melatonin could alter blood melatonin levels of the indole as well as provide protection of macromolecules against oxidative damage.