Immobilization of fibronectin in chitosan substrates improves cell adhesion and proliferation
Immobilization of fibronectin in chitosan substrates improves cell adhesion and proliferation
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DOI：
10.1002/term.248
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发表时间：
2010-06-01
影响因子：
3.3
通讯作者：
Mano, J. F.
中科院分区：
文献类型：
--
作者：
Custodio, C. A.;Alves, C. M.;Mano, J. F.
生物分子共价接枝是提高材料生物相容性和生物活性的一种策略。然而，在生物分子附着到表面后保持其生物活性至关重要。在本研究中，我们使用两种方法比较了表面富含纤连蛋白 (Fn) 的壳聚糖的生物学特性：化学固定，使用水溶性碳二亚胺；以及化学固定，使用水溶性碳二亚胺。和简单的吸附。 X 射线光电子能谱研究证实 Fn 成功固定到改性膜上。将 SaOs-2 细胞接种到这些表面上以评估此类修饰的生物学后果。 Fn 的存在刺激细胞在壳聚糖上的粘附。结果发现，在共价连接的 Fn 存在下培养 7 天后，细胞融合；在未修饰的薄膜和吸附了 Fn 的薄膜中检测到的细胞明显较少。该结果与 Fn 在培养基中 24 小时内从壳聚糖中发生大量解吸的事实相一致。这项研究表明，Fn 可以很容易地共价连接到壳聚糖基质上，从而改善材料的生物性能。该技术可以在组织工程策略中找到应用，因为基于壳聚糖的基材的表面修饰可以在更复杂的几何形状中进行，例如在支架或颗粒中。版权所有 (C) 2010 约翰·威利父子有限公司
Covalent grafting of biomolecules is a strategy to improve the biocompatibility and bioactivity of materials. However, it is critical to maintain the biological activity of the biomolecule upon its attachment to the surface. In the present study we compared the biological properties of chitosan, in which the surface was enriched with fibronectin (Fn), using two methodologies: chemical immobilization, using a water-soluble carbodiimide; and simple adsorption. X-ray photoelectron spectroscopy studies confirmed the successful immobilization of Fn onto modified membranes. SaOs-2 cells were seeded onto these surfaces to assess the biological consequences of such modifications. The presence of Fn stimulated cell adhesion on chitosan. It was found that after 7 days of culture in the presence of covalently attached Fn, the cells are confluent; significantly fewer cells were detected in unmodified film and in film with adsorbed Fn. This result is consistent with the fact that considerable desorption of Fn from chitosan takes place within 24 h in culture medium. This study showed that Fn may be easily covalently attached onto chitosan substrates, improving the biological performance of the material. The technique could find applications in tissue-engineering strategies, as the surface modification of chitosan-based substrates could be carried out in more complex geometries, such as in scaffolds or particles. Copyright (C) 2010 John Wiley & Sons, Ltd.