Correlating carbon monoxide oxidation with cox genes in the abundant Marine Roseobacter Clade
Correlating carbon monoxide oxidation with cox genes in the abundant Marine Roseobacter Clade
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DOI：
10.1038/ismej.2010.170
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发表时间：
2011-04-01
期刊：
影响因子：
11
通讯作者：
Cunliffe, Michael
中科院分区：
文献类型：
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作者：
Cunliffe, Michael
海洋玫瑰杆菌分支(MRC)是浮游细菌中具有重要数量和生物地球化学意义的组成部分。对多个MRC基因组的注释表明，存在丰富的一氧化碳脱氢酶(CODH)COX基因，这意味着MRC在海洋CO循环中发挥了作用。根据coxL基因的序列分析，COX基因分为两种不同的形式：形式I和形式II。这两种形式在MRC基因组中分布不均。大多数(18/29)的MRC基因组只含有推测的II型coxL基因。在分析的29个MRC基因组中，只有10个同时具有推定的形式II和确定的形式I coxL。没有一个只有形式I coxL。以前被证明是I型CODH酶翻译后成熟所需的基因在只包含II型的MRC基因组中缺失。随后对9株MRC菌株的子集进行的分析表明，只有同时具有两种COXL型的MRC菌株才能氧化CO。《ISME期刊》(2011年)5685-691；doi：10.1038/ismej.2010.170；2010年11月11日在线出版
The Marine Roseobacter Clade (MRC) is a numerically and biogeochemically significant component of the bacterioplankton. Annotation of multiple MRC genomes has revealed that an abundance of carbon monoxide dehydrogenase (CODH) cox genes are present, subsequently implying a role for the MRC in marine CO cycling. The cox genes fall into two distinct forms based on sequence analysis of the coxL gene; forms I and II. The two forms are unevenly distributed across the MRC genomes. Most (18/29) of the MRC genomes contain only the putative form II coxL gene. Only 10 of the 29 MRC genomes analysed have both the putative form II and the definitive form I coxL. None have only the form I coxL. Genes previously shown to be required for post-translational maturation of the form I CODH enzyme are absent from the MRC genomes containing only form II. Subsequent analyses of a subset of nine MRC strains revealed that only MRC strains with both coxL forms are able to oxidise CO. The ISME Journal (2011) 5, 685-691; doi:10.1038/ismej.2010.170; published online 11 November 2010