Combination of Weight-Bearing Training and Anti-MSTN Polyclonal Antibody Improve Bone Quality In Rats
Combination of Weight-Bearing Training and Anti-MSTN Polyclonal Antibody Improve Bone Quality In Rats
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负重训练与抗MSTN多克隆抗体相结合改善大鼠骨质量
DOI：
10.1123/ijsnem.2015-0337
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发表时间：
2016-12-01
影响因子：
2.5
通讯作者：
Sun, Lijun
中科院分区：
文献类型：
--
作者：
Tang, Liang;Gao, Xiaohang;Sun, Lijun
负重运动有益于骨骼健康。肌肉生长抑素(MSTN)缺乏对骨形成有积极作用。我们想知道，负重训练和MSTN多克隆抗体(MsAb)的结合是否会比单一治疗更大程度地促进骨形成。本研究将大鼠随机分为4组：对照组、负重训练组、负重训练组和负重训练组+负重训练组。训练后的大鼠以体重的35%以15m/min的速度跑步，40min/d(跑步2min后休息2min)，每周6d，共8周。MsAb组大鼠每周注射1次MsAb，连续8周。MicroCT分析显示，与MsAb组相比，WT+MsAb组的骨密度(BMD)、骨体积/总体积(Bv/Tv)(p<；.01)、骨小梁厚度(p<；.05)、骨小梁间距(Tb.Sp)均显著增加(p<；.01)。与WT组比较，WT+MsAb组骨小梁BMD(p<；.05)、Bv/Tv(p<；.05)显著升高，Tb.Sp(p<；.05)显著降低。三点弯曲试验表明，MsAb不能改善骨的生物力学性能(p>；.05)，负重训练显著增加能量吸收(p<；.05)和弹性模量(p<；.05)。然而，当它们结合在一起时，包括最大载荷(p<；.05)、硬度(p<；.05)、弹性模数(p<；.01)和能量吸收(p<；.01)在内的生物力学性能都显著提高。结论：负重训练联合MsAb对骨骼的积极作用大于单独应用MsAb或单独进行负重训练，提示阻力训练联合MSTN拮抗剂可能是改善骨骼健康、降低骨质疏松风险的有效途径。
Weight-bearing exercise is beneficial to bone health. Myostatin (MSTN) deficiency has a positive effect on bone formation. We wondered if a combination of weight-bearing training and polyclonal antibody for MSTN (MsAb) would augment bone formation to a greater degree than single treatment. In this study, rats were randomly assigned to four groups: Control, weight-bearing training (WT), MsAb, and WT+MsAb. The trained rats ran at 15 m/min bearing with 35% of their body weight, 40 min/day (2 min of running followed by 2 min of rest), 6 days/week, for 8 weeks. The rats with MsAb were injected once a week with MsAb for 8 weeks. MicroCT analysis showed that compared with the MsAb group, WT+MsAb significantly enhanced cortical bone mineral density (BMD) (p < .01), bone volume over total volume (BV/TV) (p < .01), trabecular thickness (p < .05), and reduced trabecular separation (Tb.Sp) (p < .01). Compared with the WT group, WT+MsAb significantly increased trabecular BMD (p < .05), BV/TV (p < .05), and decreased Tb.Sp (p < .05). Three-point bending test demonstrated that MsAb failed to improve bone biomechanical properties (p > .05), weight-bearing training significantly increased energy absorption (p < .05) and elastic modulus (p < .05). However, when they combined, biomechanical properties including maximum load (p < .05), stiffness (p < .05), elastic modulus (p < .01) and energy absorption (p < .01) were all significantly enhanced. In conclusion, the combination of weight-bearing training and MsAb have a greater positive effect on bone than treatment with either MsAb or weight-bearing training alone, suggesting that resistance training in combination with MSTN antagonists could be an effective approach for improving bone health and reducing osteoporosis risk.