Mugwort and sage (Artemisia) pollen cross-reactivity: ELISA inhibition and immunoblot evaluation
Mugwort and sage (Artemisia) pollen cross-reactivity: ELISA inhibition and immunoblot evaluation
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DOI：
10.1016/s1081-1206(10)63025-6
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发表时间：
1997-10-01
影响因子：
5.9
通讯作者：
Weber, RW
中科院分区：
文献类型：
--
作者：
Katial, RK;Lin, FL;Weber, RW
背景：蒿属植物是秋季过敏症状的来源，特别是在美国西部。研究已经确定了主要物种之一（A.目的：研究9种蒿属植物：冷蒿A，冷蒿A. annua，A. biennis、黑腹滨藜A. filifolia，A. tridentata，三齿拟步行虫A. californica，平突草A. ludoviciana和A. vulgaris.Methods:结果：酶联免疫吸附试验（ELISA）抑制剂和免疫印迹技术检测到9种蒿属过敏原的交叉反应。biennis和A. tridentata是两种最强的抑制剂。聚丙烯酰胺凝胶电泳结果表明，9种植物的电泳带型具有很大的相似性。硝酸纤维素印迹显示出类似的IgE结合模式蒿属物种之间的强烈抑制所有9个extract.Conclusions：这些数据都表现出非常强的体外交叉反应的9个蒿属物种之间的研究。这些数据具有显著的临床相关性，表明单一蒿属物种可能足以用于过敏皮肤测试和免疫治疗提取物的配制。
Background: Plants of the genus Artemisia are a source of fall allergic symptoms, particularly in the western United States. Studies have characterized the allergens in one of the major species (A. vulgaris) but currently there are no cross-reactivity data on the major United States species.Objective: The purpose of this study was to investigate the in vitro cross-reactivity among nine Artemisia species: A, frigida, A. annua, A. biennis, A. filifolia, A. tridentata, A. californica, A, gnaphalodes, A. ludoviciana, and A. vulgaris.Methods: The cross-reactivity was demonstrated with the use of enzyme-linked immunosorbent assay (ELISA) inhibitions and immunoblotting techniques utilizing a serum pool from patients allergic Co Artemisia species.Results: The enzyme-linked immunosorbent assay inhibitions revealed strong cross-reactivity among all nine species with A. biennis and A. tridentata being two of the strongest inhibitors. The polyacrylamide gel electrophoresis showed a great deal of similarity in the bands among the nine species. The nitrocellulose blots showed similar IgE binding patterns among the Artemisia species with strong inhibition among all nine extracts.Conclusions: These data all demonstrate very strong in vitro cross-reactivity among the nine Artemisia species studied. Such data have significant clinical relevance, suggesting that a single Artemisia species may be sufficient for allergy skin testing and formulation of immunotherapy extracts.