INTERRELATIONSHIP OF REPAIR MECHANISMS IN ULTRAVIOLET-IRRADIATED ESCHERICHIA-COLI
INTERRELATIONSHIP OF REPAIR MECHANISMS IN ULTRAVIOLET-IRRADIATED ESCHERICHIA-COLI
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DOI：
10.1128/jb.120.1.15-23.1974
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发表时间：
1974-01-01
影响因子：
3.2
通讯作者：
DAVIES, DJG
中科院分区：
文献类型：
--
作者：
MOSS, SH;DAVIES, DJG
调查的影响，光复活，切除，重组修复，单独和组合，对紫外线照射的大肠杆菌K-12突变体的生存导致了一个可能的解释的损失的光反应性和复杂的变化，在液体保持期间观察到的生存能力。有人建议，在较高的紫外光剂量的切除修复机制变得饱和，由于重叠的切除区域上的相对链的脱氧核糖核酸螺旋。研究结果还支持现有的假设，即紫外线照射的细菌的肩状存活曲线的形状可以描述的重叠切除区域的发生概率。使用与修复缺陷突变体密切相关的遗传组成获得的数据，我们提出了一个数学模型，准确地预测观察到的生存曲线的形状，并提供了切除修复机制删除的脱氧核糖核酸的每个片段中的核苷酸数量的估计。
Investigation of the effect of photoreactivation, excision, and recombination repair, individually and in combination, on the survival of ultraviolet-irradiatedEscherichia coliK-12 mutants has led to a possible explanation of the loss of photoreactivability and of the complex changes in viability observed during liquid-holding. It is suggested that at higher ultraviolet light doses the excision repair mechanism becomes saturated due to overlapping of excised regions on opposite strands of the deoxyribonucleic acid helix. The results also provide support for the existing hypothesis that states that the shape of shouldered survival curves of ultraviolet-irradiated bacteria can be described in terms of the probability of occurrance of overlapping excised regions. Using the data obtained with repair-deficient mutants with closely related genetic makeup, we present a mathematical model that accurately predicts the shape of the observed survival curves and provides an estimate of the number of nucleotides in each fragment of deoxyribonucleic acid removed by the excision repair mechanism.