Hypermethylated SUPERMAN epigenetic alleles in Arabidopsis
Hypermethylated SUPERMAN epigenetic alleles in Arabidopsis
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DOI：
10.1126/science.277.5329.1100
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发表时间：
1997-08-22
期刊：
影响因子：
56.9
通讯作者：
Meyerowitz, EM
中科院分区：
文献类型：
--
作者：
Jacobsen, SE;Meyerowitz, EM
超人基因的突变影响拟南芥花的发育。七个可遗传但不稳定的sup表观等位基因（clark kent等位基因）与sup基因中过量胞嘧啶甲基化和sup RNA水平降低的几乎相同的模式相关。这些等位基因的复生体在SUP位点被大量去甲基化，并恢复了SUP RNA的水平。一个携带反义甲基转移酶基因的转基因拟南芥品系，其基因组胞嘧啶甲基化总体下降，也含有一个高甲基化的sup等位基因。因此，甲基化系统的破坏可能产生比预期更复杂的结果，并可能导致已知基因的甲基化缺陷。克拉克肯特等位基因不同于反义系，因为它们没有显示出基因组甲基化的普遍减少。
Mutations in the SUPERMAN gene affect flower development in Arabidopsis. Seven heritable but unstable sup epi-alleles (the clark kent alleles) are associated with nearly identical patterns of excess cytosine methylation within the SUP gene and a decreased level of SUP RNA. Revertants of these alleles are largely demethylated at the SUP locus and have restored levels of SUP RNA. A transgenic Arabidopsis line carrying an antisense methyltransferase gene, which shows an overall decrease in genomic cytosine methylation, also contains a hypermethylated sup allele. Thus, disruption of methylation systems may yield more complex outcomes than expected and can result in methylation defects at known genes. The clark kent alleles differ from the antisense line because they do not show a general decrease in genomic methylation.