Postnatal mouse subventricular zone neuronal precursors can migrate and differentiate within multiple levels of the developing neuraxis.
Postnatal mouse subventricular zone neuronal precursors can migrate and differentiate within multiple levels of the developing neuraxis.
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DOI：
10.1073/pnas.94.26.14832
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发表时间：
1997-12
影响因子：
11.1
通讯作者：
Daniel A. Lim;Gord Fishell;A. Alvarez-Buylla
中科院分区：
文献类型：
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作者：
Daniel A. Lim;Gord Fishell;A. Alvarez-Buylla
哺乳动物前脑室外侧壁的室下区（SVZ）一生中保留着大量增殖的神经元前体。出生后和成年SVZ的神经元前体迁移到嗅球，在那里它们分化成中间神经元。在这里，我们测试了小鼠出生后SVZ前体在胚胎脑环境中的潜能：(i)普遍存在的遗传标记，（ii）神经元特异性转基因，以及（iii）亲脂染料用于跟踪在妊娠第15天移植到胚胎小鼠脑室的出生后5-10天SVZ细胞的命运。移植物源性细胞分布于神经轴的多个层次，包括中隔、丘脑、下丘脑，中脑下丘也有大量移植物源性细胞。我们没有观察到大脑皮层的整合。通过神经元特异性β -微管蛋白抗体的双染色、神经元特异性转基因的表达和亲脂染料显示的树突状乔木，证实了移植物来源细胞的神经元分化。我们的结论是，出生后的SVZ细胞可以迁移并分化为胚胎大脑的多个区域内的神经元，而不是嗅球。
The mammalian subventricular zone (SVZ) of the lateral wall of the forebrain ventricle retains a population of proliferating neuronal precursors throughout life. Neuronal precursors born in the postnatal and adult SVZ migrate to the olfactory bulb where they differentiate into interneurons. Here we tested the potential of mouse postnatal SVZ precursors in the environment of the embryonic brain: (i) a ubiquitous genetic marker, (ii) a neuron-specific transgene, and (iii) a lipophilic-dye were used to follow the fate of postnatal day 5-10 SVZ cells grafted into embryonic mouse brain ventricles at day 15 of gestation. Graft-derived cells were found at multiple levels of the neuraxis, including septum, thalamus, hypothalamus, and in large numbers in the midbrain inferior colliculus. We observed no integration into the cortex. Neuronal differentiation of graft derived cells was demonstrated by double-staining with neuron-specific beta-tubulin antibodies, expression of the neuron-specific transgene, and the dendritic arbors revealed by the lipophilic dye. We conclude that postnatal SVZ cells can migrate through and differentiate into neurons within multiple embryonic brain regions other than the olfactory bulb.